TY - JOUR
T1 - In Situ Characterization of the Protein Corona of Nanoparticles In Vitro and In Vivo
AU - Latreille, Pierre-Luc
AU - Rabanel, Jean-Michel
AU - Le Goas, Marine
AU - Salimi, Sina
AU - Arlt, Jochen
AU - Patten, Shunmoogum A.
AU - Ramassamy, Charles
AU - Hildgen, Patrice
AU - Martinez, Vincent A.
AU - Banquy, Xavier
N1 - Funding Information:
Charlotte Zaouter (Pr SA Patten laboratory) is acknowledged for her help with zebrafish husbandry. The authors would also like to thank Jessy Tremblay, coordinator of the cytometry and microscopy platform at the INRS Center Armand‐Frappier Santé Biotechnologie, for his help with the in vivo microscopy experiments. P.L.L. thanks NSERC/CRSNG (Government of Canada) and faculty of pharmacy (UdeM) for Ph.D. scholarship. J.M.R. thanks NSERC/CRSNG (Government of Canada) for postdoctoral fellowship. This research was undertaken thanks, in part, to funding from the Canada First Research Excellence Fund through the TransMedTech Institute (postdoctoral fellowships given to J.M.R. and M.L.G., doctoral grant to S.S.). X.B. is grateful to the Canada Research Chair funding program and NSERC (Discovery grant and NOVA) for financial support.
Publisher Copyright:
© 2022 The Authors. Advanced Materials published by Wiley-VCH GmbH.
PY - 2022/7/28
Y1 - 2022/7/28
N2 - A new theoretical framework that enables the use of differential dynamic microscopy (DDM) in fluorescence imaging mode to quantify in situ protein adsorption onto nanoparticles (NP) while simultaneously monitoring for NP aggregation is proposed. This methodology is used to elucidate the thermodynamic and kinetic properties of the protein corona (PC) in vitro and in vivo. The results show that protein adsorption triggers particle aggregation over a wide concentration range and that the formed aggregate structures can be quantified using the proposed methodology. Protein affinity for polystyrene (PS) NPs is observed to be dependent on particle concentration. For complex protein mixtures, this methodology identifies that the PC composition changes with the dilution of serum proteins, demonstrating a Vroman effect never quantitatively assessed in situ on NPs. Finally, DDM allows monitoring of the evolution of the PC in vivo. This results show that the PC composition evolves significantly over time in zebrafish larvae, confirming the inherently dynamic nature of the PC. The performance of the developed methodology allows to obtain quantitative insights into nano-bio interactions in a vast array of physiologically relevant conditions that will serve to further improve the design of nanomedicine.
AB - A new theoretical framework that enables the use of differential dynamic microscopy (DDM) in fluorescence imaging mode to quantify in situ protein adsorption onto nanoparticles (NP) while simultaneously monitoring for NP aggregation is proposed. This methodology is used to elucidate the thermodynamic and kinetic properties of the protein corona (PC) in vitro and in vivo. The results show that protein adsorption triggers particle aggregation over a wide concentration range and that the formed aggregate structures can be quantified using the proposed methodology. Protein affinity for polystyrene (PS) NPs is observed to be dependent on particle concentration. For complex protein mixtures, this methodology identifies that the PC composition changes with the dilution of serum proteins, demonstrating a Vroman effect never quantitatively assessed in situ on NPs. Finally, DDM allows monitoring of the evolution of the PC in vivo. This results show that the PC composition evolves significantly over time in zebrafish larvae, confirming the inherently dynamic nature of the PC. The performance of the developed methodology allows to obtain quantitative insights into nano-bio interactions in a vast array of physiologically relevant conditions that will serve to further improve the design of nanomedicine.
KW - bio-nano interactions
KW - differential dynamic microscopy
KW - in vivo quantification
KW - protein corona
KW - Vroman effect
U2 - 10.1002/adma.202203354
DO - 10.1002/adma.202203354
M3 - Article
SN - 0935-9648
VL - 2022
SP - 1
EP - 14
JO - Advanced Materials
JF - Advanced Materials
M1 - 2203354
ER -