In situ hybridization as a tool to study the role of microRNAs in plant development

Catherine Kidner*, Marja Timmermans

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

Abstract

MicroRNAs (miRNAs) have a vital role in the generation of plant forms through post-transcriptional regulation of the accumulation of developmental regulators. Analysis of their roles requires detailed knowledge of their expression patterns. We describe an in situ hybridization technique we have used to study the patterns of miRNA accumulation in Arabidopsis and in maize.

Original languageEnglish
Pages (from-to)159-179
Number of pages21
JournalMethods in molecular biology (Clifton, N.J.)
Volume342
DOIs
Publication statusPublished - 1 Jan 2006

Keywords

  • in situ hybridisation
  • microRNA
  • plants

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