In situ hybridization of corticotropin-releasing factor-encoding messenger RNA in the hypothalamus of the white sucker, Catostomus commersoni

Y Okawara, D Ko, S D Morley, D Richter, K P Lederis

Research output: Contribution to journalArticlepeer-review

Abstract

In situ hybridization procedure with a 32P-labelled synthetic oligonucleotide probe was used to detect corticotropin-releasing factor-encoding messenger RNA (CRF mRNA) in the hypothalamus of the white sucker, Catostomus commersoni. Adjacent sections were immunostained by a sucker CRF-specific antiserum. CRF mRNA-containing neurons were identified by autoradiography in the magnocellular and parvocellular subdivisions of the preoptic nucleus (PON). Many of these neurons were also immunostained by sucker antiserum, showing the same distribution patterns. These results confirm the presence of CRF mRNA and CRF peptide in the white sucker hypothalamus and support the view that the magnocellular and parvocellular neurons of the PON may be involved in the control of adrenocorticotropic hormone secretion from the pituitary in the white sucker.

Original languageEnglish
Pages (from-to)545-9
Number of pages5
JournalCell and tissue research
Volume267
Issue number3
Publication statusPublished - Mar 1992

Keywords

  • Animals
  • Autoradiography
  • Base Sequence
  • Corticotropin-Releasing Hormone
  • Female
  • Fishes
  • Hypothalamus
  • Immune Sera
  • Immunohistochemistry
  • Male
  • Molecular Sequence Data
  • Neurons
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes
  • Phosphorus Radioisotopes
  • Preoptic Area
  • RNA, Messenger

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