Projects per year
Abstract / Description of output
Respiratory infections in mechanically ventilated patients caused by Gram-negative bacteria are a major cause of morbidity. Rapid and unequivocal determination of the presence, localization, and abundance of bacteria is critical for positive resolution of the infections and could be used for patient stratification and for monitoring treatment efficacy. Here, we developed an in situ approach to visualize Gram-negative bacterial species and cellular infiltrates in distal human lungs in real time. We used optical endomicroscopy to visualize a water-soluble optical imaging probe based on the antimicrobial peptide polymyxin conjugated to an environmentally sensitive fluorophore. The probe was chemically stable and nontoxic and, after in-human intrapulmonary microdosing, enabled the specific detection of Gram-negative bacteria in distal human airways and alveoli within minutes. The results suggest that pulmonary molecular imaging using a topically administered fluorescent probe targeting bacterial lipid A is safe and practical, enabling rapid in situ identification of Gram-negative bacteria in humans.
Original language | English |
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Article number | eaal0033 |
Number of pages | 12 |
Journal | Science Translational Medicine |
Volume | 10 |
Issue number | 464 |
Early online date | 24 Oct 2018 |
DOIs | |
Publication status | Published - 24 Oct 2018 |
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Dive into the research topics of 'In situ identification of Gram-negative bacteria in human lungs using a topical fluorescent peptide targeting lipid A'. Together they form a unique fingerprint.Projects
- 1 Finished
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Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging
Bradley, M.
1/10/13 → 31/03/19
Project: Research
Profiles
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David Collie
- Royal (Dick) School of Veterinary Studies - Group Leader/Reader
- Edinburgh Imaging
Person: Academic: Research Active
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Kevin Dhaliwal
- Deanery of Clinical Sciences - Personal Chair of Molecular Imaging & Healthcare
- Centre for Inflammation Research
- Edinburgh Imaging
Person: Academic: Research Active , Academic: Research Active (Research Assistant)
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