In Situ Nanoliter-Scale Polymer Fabrication for Flexible Cell Patterning

Albert R. Liberski; Rong Zhang; Mark Bradley

doi:10.1016/j.jala.2009.03.005

In Situ Nanoliter-Scale Polymer Fabrication for Flexible Cell Patterning

Albert R. Liberski, Rong Zhang, Mark Bradley

Research output: Contribution to journal › Article › peer-review

Abstract

Drug-testing technologies, biosensor fabrication, tissue engineering, and basic biological research depend strongly on the patterning of live animal cells. Current techniques for controlling cellular adhesion are restricted with two primary limitations. Firstly, the complexity of the available patterns is very limited and, secondly, the pallet of materials that induce cellular patterning is exhaustible. Here, we demonstrate a method for computer-aided control of cell patterning using a scientific inkjet printer that yields a highly complex cellular pattern suitable for applications in regenerative medicine and rapid prototyping, and a strategy for using in situ polymerization for fabrication polymeric patterns directly on-chip. (JALA 2009;14:285-93)

Original language	English
Pages (from-to)	285-293
Number of pages	9
Journal	Journal of The Association for Laboratory Automation
Volume	14
Issue number	5
DOIs	https://doi.org/10.1016/j.jala.2009.03.005
Publication status	Published - Oct 2009

Keywords

cell patterning
software for inkjet printing
mES cells
biomaterials
nL scale-in situ polymerization
synthetic cell binders
SELF-ASSEMBLED MONOLAYERS
DIP-PEN NANOLITHOGRAPHY
EMBRYONIC STEM-CELLS
DISSOCIATED NEURONS
MICROARRAYS
SURFACES
ADHESION
DIFFERENTIATION
CULTURE
MICROENVIRONMENT

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10.1016/j.jala.2009.03.005

Cite this

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title = "In Situ Nanoliter-Scale Polymer Fabrication for Flexible Cell Patterning",

abstract = "Drug-testing technologies, biosensor fabrication, tissue engineering, and basic biological research depend strongly on the patterning of live animal cells. Current techniques for controlling cellular adhesion are restricted with two primary limitations. Firstly, the complexity of the available patterns is very limited and, secondly, the pallet of materials that induce cellular patterning is exhaustible. Here, we demonstrate a method for computer-aided control of cell patterning using a scientific inkjet printer that yields a highly complex cellular pattern suitable for applications in regenerative medicine and rapid prototyping, and a strategy for using in situ polymerization for fabrication polymeric patterns directly on-chip. (JALA 2009;14:285-93)",

keywords = "cell patterning, software for inkjet printing, mES cells, biomaterials, nL scale-in situ polymerization, synthetic cell binders, SELF-ASSEMBLED MONOLAYERS, DIP-PEN NANOLITHOGRAPHY, EMBRYONIC STEM-CELLS, DISSOCIATED NEURONS, MICROARRAYS, SURFACES, ADHESION, DIFFERENTIATION, CULTURE, MICROENVIRONMENT",

author = "Liberski, {Albert R.} and Rong Zhang and Mark Bradley",

year = "2009",

month = oct,

doi = "10.1016/j.jala.2009.03.005",

language = "English",

volume = "14",

pages = "285--293",

journal = "Journal of The Association for Laboratory Automation",

issn = "1535-5535",

publisher = "SAGE Publications",

number = "5",

}

TY - JOUR

T1 - In Situ Nanoliter-Scale Polymer Fabrication for Flexible Cell Patterning

AU - Liberski, Albert R.

AU - Zhang, Rong

AU - Bradley, Mark

PY - 2009/10

Y1 - 2009/10

N2 - Drug-testing technologies, biosensor fabrication, tissue engineering, and basic biological research depend strongly on the patterning of live animal cells. Current techniques for controlling cellular adhesion are restricted with two primary limitations. Firstly, the complexity of the available patterns is very limited and, secondly, the pallet of materials that induce cellular patterning is exhaustible. Here, we demonstrate a method for computer-aided control of cell patterning using a scientific inkjet printer that yields a highly complex cellular pattern suitable for applications in regenerative medicine and rapid prototyping, and a strategy for using in situ polymerization for fabrication polymeric patterns directly on-chip. (JALA 2009;14:285-93)

AB - Drug-testing technologies, biosensor fabrication, tissue engineering, and basic biological research depend strongly on the patterning of live animal cells. Current techniques for controlling cellular adhesion are restricted with two primary limitations. Firstly, the complexity of the available patterns is very limited and, secondly, the pallet of materials that induce cellular patterning is exhaustible. Here, we demonstrate a method for computer-aided control of cell patterning using a scientific inkjet printer that yields a highly complex cellular pattern suitable for applications in regenerative medicine and rapid prototyping, and a strategy for using in situ polymerization for fabrication polymeric patterns directly on-chip. (JALA 2009;14:285-93)

KW - cell patterning

KW - software for inkjet printing

KW - mES cells

KW - biomaterials

KW - nL scale-in situ polymerization

KW - synthetic cell binders

KW - SELF-ASSEMBLED MONOLAYERS

KW - DIP-PEN NANOLITHOGRAPHY

KW - EMBRYONIC STEM-CELLS

KW - DISSOCIATED NEURONS

KW - MICROARRAYS

KW - SURFACES

KW - ADHESION

KW - DIFFERENTIATION

KW - CULTURE

KW - MICROENVIRONMENT

UR - http://www.scopus.com/inward/record.url?scp=69449094743&partnerID=8YFLogxK

U2 - 10.1016/j.jala.2009.03.005

DO - 10.1016/j.jala.2009.03.005

M3 - Article

VL - 14

SP - 285

EP - 293

JO - Journal of The Association for Laboratory Automation

JF - Journal of The Association for Laboratory Automation

SN - 1535-5535

IS - 5

ER -

In Situ Nanoliter-Scale Polymer Fabrication for Flexible Cell Patterning

Abstract

Keywords

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HT Chemical manipuation of foetal and adult stem cells - selection, tranfection and scaffold identification

Biocompatible polymer micro-arrays for cellular growth, stem cell manipulation, cellular release and identification and high-content screening

Cite this

In Situ Nanoliter-Scale Polymer Fabrication for Flexible Cell Patterning

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Projects

HT Chemical manipuation of foetal and adult stem cells - selection, tranfection and scaffold identification

Biocompatible polymer micro-arrays for cellular growth, stem cell manipulation, cellular release and identification and high-content screening

Cite this