Projects per year
Abstract / Description of output
Microbial cell factories express diverse heterologous pathways for the production of a wide range of valuable natural products. However, the recovery and purification of such compounds is a major bottleneck in commercialization. In this study, a novel in situ solid phase adsorption strategy was investigated for enhanced recovery of taxadiene, a precursor to the blockbuster anticancer drug, paclitaxel, from engineered Saccharomyces cerevisiae. A synthetic adsorbent resin (HP-20) was employed to efficiently sequester taxadiene as it was secreted during growth and a carefully optimized desorption solvent was applied following cultivation to maximize re-covery of both secreted and intracellular taxadiene, across a range of scales (2 – 250 mL). Resin concentration was found to have an impact on cellular growth, with the high concentration of 12 % (w/v) resulting in frag-mentation of the resin beads, which was detrimental to growth. The optimal resin concentration and desorption solvent combination elucidated at microscale (2 mL) resulted in a two-fold improvement in taxadiene titer to 61± 8 mg/L, compared to the traditional liquid-liquid extraction approach (dodecane overlay). Taxadiene was found to be distributed evenly between resin beads and biomass. Performance of the optimal process was subsequently investigated through scale-up using controlled mini-bioreactors (250 mL). Here, a comparable tax-adiene titer of 76 ± 19 mg/L was achieved despite a 125-fold scale-up in cultivation volume. This represented a1.4-fold improvement in taxadiene recovery compared to previous mini-bioreactor scale cultivations using the dodecane overlay extraction approach.
Original language | English |
---|---|
Article number | 120880 |
Number of pages | 11 |
Journal | Separation and Purification Technology |
Volume | 290 |
DOIs | |
Publication status | Published - 1 Jun 2022 |
Keywords / Materials (for Non-textual outputs)
- taxadiene
- in situ solid phase adsorption
- mini bioreactor
- HP 20 resin beads
- saccharomyces cerevisiae
Fingerprint
Dive into the research topics of 'In situ solid-liquid extraction enhances recovery of taxadiene from engineered Saccharomyces cerevisiae cell factories'. Together they form a unique fingerprint.Projects
- 2 Finished
-
Metabolic Engineering of Yarrowia lipolytica to produce an advanced biofuel from cellulose and plant wastes
Rios Solis, L.
UK central government bodies/local authorities, health and hospital authorities
1/08/20 → 30/03/23
Project: Research
-
A sustainable production platform for industrially relevant chemicals
Loake, G.
1/09/18 → 31/10/20
Project: Research