Projects per year
In vitro histone modification (HM) assays are used to characterize the activity of chromatin-modifying enzymes. These assays provide information regarding the modification sites on histones, the product specificity, and the impact of other histone or nucleotide modifications on enzyme activity. In particular, histone methyltransferase (HMT) assays have been instrumental in elucidating the activity and site specificity of many plant HMT enzymes. In this chapter, we describe a general protocol that can be used to perform HMT assays using different chromatin substrates, detection methods, and enzymes directly purified from plant material or heterologous sources.
|Title of host publication||Plant Chromatin Dynamics|
|Editors||Marian Bemer, Célia Baroux|
|Publisher||HUMANA PRESS INC|
|Number of pages||16|
|Edition||1st ed. 2018 edition|
|ISBN (Print)||1493973177, 9781493973170|
|Publication status||Published - 20 Oct 2017|
|Name||Methods in Molecular Biology|
- Histone modifications
- histone variants
- histone lysine methylation
- histone peptides
- histone octamers
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- 2 Finished
Roles of symmetric and asymmetric histone H3 lysine 27 trimethylation in gene repression and epigenetic inheritance
Voigt, P. & Finnegan, D.
1/11/14 → 31/12/21
Core funding renewal for the Wellcome Trust Centre for Cell Biology
1/10/11 → 30/04/17