Abstract / Description of output
Objective: To determine the in-vitro compatibility of rabbit and canine blood using both a tube and slide agglutination crossmatch technique and to compare the results obtained from these two methods.
Design: Prospective observational laboratory study from January-March 2020.
Setting: University veterinary teaching hospital.
Animals: Six client owned rabbits ≥3.5kg undergoing phlebotomy for a primary clinical reason. “Pigtail’’ blood samples from three DEA 1 positive and three DEA 1 negative canine packed red blood cell units.
Interventions: Blood from each rabbit was crossmatched with a single unit of canine blood using both a standard laboratory tube agglutination technique and a simple slide agglutination method with each rabbit/canine unit serving as its own intra-assay control. Tube crossmatches were evaluated for agglutination both macro-and microscopically and assessed for hemolysis. Slide crossmatches were assessed for the presence of agglutination both macro- and microscopically.
Measurements and Main Results: All crossmatches were incompatible. Varying degrees of agglutination was seen for all crossmatches. Hemolysis was observed with all minor tube crossmatches. Results of both crossmatch techniques were in close agreement.
Conclusions: The crossmatch results in this present study strongly demonstrates in-vitro incompatibility between canine and rabbit blood. Agreement between the two techniques in this study indicates that the slide agglutination technique may be quicker, require less blood and provide reliable results in exclusively assessing the compatibility of canine and rabbit blood. Based on the results of this study, emergency xenotransfusion of canine blood to rabbits
Design: Prospective observational laboratory study from January-March 2020.
Setting: University veterinary teaching hospital.
Animals: Six client owned rabbits ≥3.5kg undergoing phlebotomy for a primary clinical reason. “Pigtail’’ blood samples from three DEA 1 positive and three DEA 1 negative canine packed red blood cell units.
Interventions: Blood from each rabbit was crossmatched with a single unit of canine blood using both a standard laboratory tube agglutination technique and a simple slide agglutination method with each rabbit/canine unit serving as its own intra-assay control. Tube crossmatches were evaluated for agglutination both macro-and microscopically and assessed for hemolysis. Slide crossmatches were assessed for the presence of agglutination both macro- and microscopically.
Measurements and Main Results: All crossmatches were incompatible. Varying degrees of agglutination was seen for all crossmatches. Hemolysis was observed with all minor tube crossmatches. Results of both crossmatch techniques were in close agreement.
Conclusions: The crossmatch results in this present study strongly demonstrates in-vitro incompatibility between canine and rabbit blood. Agreement between the two techniques in this study indicates that the slide agglutination technique may be quicker, require less blood and provide reliable results in exclusively assessing the compatibility of canine and rabbit blood. Based on the results of this study, emergency xenotransfusion of canine blood to rabbits
| Original language | English |
|---|---|
| Journal | Journal of Veterinary Emergency and Critical Care |
| Early online date | 17 Oct 2021 |
| DOIs | |
| Publication status | E-pub ahead of print - 17 Oct 2021 |
Keywords / Materials (for Non-textual outputs)
- Agglutination
- Crossmatch
- Xenotransfusion