Abstract / Description of output
Purpose: To test the effect of cyclic adenosine 3′, 5′-monophosphate (cAMP) on early human ovarian follicles during prolonged culture period.
Methods: Donated ovarian biopsies from 16 women undergoing gynecological laparoscopy were cut into slices and cultured in parallel for 1, 2, or 3 weeks in the presence and the absence of 0.5 mM 8-bromo-cAMP. The developmental stages, sizes, and viability of the follicles were recorded from histological sections of all samples.
Results: On day 14, cortical slices cultured with 8-bromo-cAMP showed a significantly higher proportion of secondary follicles (50.0% vs. 20.0%) and a lower proportion of primordial follicles (9.7% vs. 26.7%) when compared with those cultured without 8-bromo-cAMP. On day 21, the proportion of viable follicles in cortical slices with 8-bromo-cAMP treatment was significantly higher than that without 8-bromo-cAMP treatment (79.6% vs. 55.2%).
Conclusion: CyclicAMP promoted folliculogenesis and follicle survival during 14-21 days' culture of human ovarian cortical slices.
Original language | English |
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Pages (from-to) | 301-306 |
Number of pages | 6 |
Journal | Journal of Assisted Reproduction and Genetics |
Volume | 21 |
Issue number | 8 |
DOIs | |
Publication status | Published - Aug 2004 |
Keywords / Materials (for Non-textual outputs)
- cAMP
- human
- organ culture
- ovarian follicles