In vitro effect of cyclic adenosine 3′, 5′-monophosphate (cAMP) on early human ovarian follicles

Pu Zhang, Henna Louhio, Timo Tuuri, Jari Sjöberg, Julius Hreinsson, Evelyn E. Telfer, Outi Hovatta

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Purpose: To test the effect of cyclic adenosine 3′, 5′-monophosphate (cAMP) on early human ovarian follicles during prolonged culture period. 

Methods: Donated ovarian biopsies from 16 women undergoing gynecological laparoscopy were cut into slices and cultured in parallel for 1, 2, or 3 weeks in the presence and the absence of 0.5 mM 8-bromo-cAMP. The developmental stages, sizes, and viability of the follicles were recorded from histological sections of all samples. 

Results: On day 14, cortical slices cultured with 8-bromo-cAMP showed a significantly higher proportion of secondary follicles (50.0% vs. 20.0%) and a lower proportion of primordial follicles (9.7% vs. 26.7%) when compared with those cultured without 8-bromo-cAMP. On day 21, the proportion of viable follicles in cortical slices with 8-bromo-cAMP treatment was significantly higher than that without 8-bromo-cAMP treatment (79.6% vs. 55.2%). 

Conclusion: CyclicAMP promoted folliculogenesis and follicle survival during 14-21 days' culture of human ovarian cortical slices.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalJournal of Assisted Reproduction and Genetics
Volume21
Issue number8
DOIs
Publication statusPublished - Aug 2004

Keywords / Materials (for Non-textual outputs)

  • cAMP
  • human
  • organ culture
  • ovarian follicles

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