In vitro infection of bovine alloreactive cytotoxic T cell lines with sporozoites of Theileria annulata and T parva

E A Innes, P Millar, E J Glass, C G Brown, R L Spooner

Research output: Contribution to journalArticlepeer-review

Abstract

Bovine alloreactive cytotoxic lymphocyte (CTL) lines of known target specificity were infected in vitro with sporozoites of Theileria annulata and T parva and cultured in limiting dilution. The phenotypes of the CTL lines both pre- and post infection were assessed using a panel of monoclonal antibodies specific for defined bovine lymphocyte subpopulations. The effector function of the resultant infected cell lines was determined using a Cr51 release assay and compared to the uninfected control CTL line. The results indicated that T parva sporozoites consistently infected and transformed the CTL lines very efficiently even at the lowest cell doses. In contrast the T annulata sporozoites were largely unable to infect and transform the alloreactive CTL except at the very highest cell and sporozoite doses. A factor which appeared to influence susceptibility to T annulata infection was an increased level of class II expression on the CTL line. None of the cell lines showed cytotoxic effector function after infection with either T annulata or T parva sporozoites.
Original languageEnglish
Pages (from-to)367-74
Number of pages8
JournalResearch in Veterinary Science
Volume46
Issue number3
Publication statusPublished - May 1989

Keywords

  • Animals
  • Apicomplexa
  • Cattle
  • Cell Line
  • T-Lymphocytes, Cytotoxic
  • Theileriasis

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