In vitro whole-organ imaging: 4D quantification of growing mouse limb buds

Marit J Boot; C Henrik Westerberg; Juanjo Sanz-Ezquerro; James Cotterell; Ronen Schweitzer; Miguel Torres; James Sharpe

doi:10.1038/nmeth.1219

In vitro whole-organ imaging: 4D quantification of growing mouse limb buds

Marit J Boot, C Henrik Westerberg, Juanjo Sanz-Ezquerro, James Cotterell, Ronen Schweitzer, Miguel Torres, James Sharpe

Research output: Contribution to journal › Article › peer-review

Abstract

Quantitative mapping of the normal tissue dynamics of an entire developing mammalian organ has not been achieved so far but is essential to understand developmental processes and to provide quantitative data for computational modeling. We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT).

Original language	English
Pages (from-to)	609-612
Number of pages	4
Journal	Nature Methods
Volume	5
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.1219
Publication status	Published - Jul 2008

Keywords / Materials (for Non-textual outputs)

Animals
Body Patterning
Extremities
Gene Expression Regulation, Developmental
Green Fluorescent Proteins
Imaging, Three-Dimensional
Mice
Mice, Transgenic
Recombinant Proteins
Time Factors
Tomography, Optical

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10.1038/nmeth.1219

http://www.nature.com/nmeth/journal/v5/n7/full/nmeth.1219.html

Cite this

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title = "In vitro whole-organ imaging: 4D quantification of growing mouse limb buds",

abstract = "Quantitative mapping of the normal tissue dynamics of an entire developing mammalian organ has not been achieved so far but is essential to understand developmental processes and to provide quantitative data for computational modeling. We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT).",

keywords = "Animals, Body Patterning, Extremities, Gene Expression Regulation, Developmental, Green Fluorescent Proteins, Imaging, Three-Dimensional, Mice, Mice, Transgenic, Recombinant Proteins, Time Factors, Tomography, Optical",

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AU - Boot, Marit J

AU - Westerberg, C Henrik

AU - Sanz-Ezquerro, Juanjo

AU - Cotterell, James

AU - Schweitzer, Ronen

AU - Torres, Miguel

AU - Sharpe, James

PY - 2008/7

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N2 - Quantitative mapping of the normal tissue dynamics of an entire developing mammalian organ has not been achieved so far but is essential to understand developmental processes and to provide quantitative data for computational modeling. We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT).

AB - Quantitative mapping of the normal tissue dynamics of an entire developing mammalian organ has not been achieved so far but is essential to understand developmental processes and to provide quantitative data for computational modeling. We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT).

KW - Animals

KW - Body Patterning

KW - Extremities

KW - Gene Expression Regulation, Developmental

KW - Green Fluorescent Proteins

KW - Imaging, Three-Dimensional

KW - Mice

KW - Mice, Transgenic

KW - Recombinant Proteins

KW - Time Factors

KW - Tomography, Optical

U2 - 10.1038/nmeth.1219

DO - 10.1038/nmeth.1219

M3 - Article

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SP - 609

EP - 612

JO - Nature Methods

JF - Nature Methods

IS - 7

ER -

In vitro whole-organ imaging: 4D quantification of growing mouse limb buds

Abstract

Keywords / Materials (for Non-textual outputs)

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