In vitro whole-organ imaging: 4D quantification of growing mouse limb buds

Marit J Boot, C Henrik Westerberg, Juanjo Sanz-Ezquerro, James Cotterell, Ronen Schweitzer, Miguel Torres, James Sharpe

Research output: Contribution to journalArticlepeer-review


Quantitative mapping of the normal tissue dynamics of an entire developing mammalian organ has not been achieved so far but is essential to understand developmental processes and to provide quantitative data for computational modeling. We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT).
Original languageEnglish
Pages (from-to)609-612
Number of pages4
JournalNature Methods
Issue number7
Publication statusPublished - Jul 2008


  • Animals
  • Body Patterning
  • Extremities
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins
  • Imaging, Three-Dimensional
  • Mice
  • Mice, Transgenic
  • Recombinant Proteins
  • Time Factors
  • Tomography, Optical


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