In vivo Analysis of White Adipose Tissue in Zebrafish

James E N Minchin; John F. Rawls

doi:10.1016/B978-0-12-381320-6.00003-5

In vivo Analysis of White Adipose Tissue in Zebrafish

James E N Minchin^*, John F. Rawls

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

White adipose tissue (WAT) is the major site of energy storage in bony vertebrates, and also serves central roles in the endocrine regulation of energy balance. The cellular and molecular mechanisms underlying WAT development and physiology are not well understood. This is due in part to difficulties associated with imaging adipose tissues in mammalian model systems, especially during early life stages. The zebrafish (Danio rerio) has recently emerged as a new model system for adipose tissue research, in which WAT can be imaged in a transparent living vertebrate at all life stages. Here we present detailed methods for labeling adipocytes in live zebrafish using fluorescent lipophilic dyes, and for in vivo microscopy of zebrafish WAT.

Original language	English
Pages (from-to)	63-86
Number of pages	24
Journal	Methods in cell biology
Volume	105
DOIs	https://doi.org/10.1016/B978-0-12-381320-6.00003-5
Publication status	Published - 2011

Keywords / Materials (for Non-textual outputs)

Adipocyte
Adipose
Cellularity
Fluorescent
Imaging
Postembryonic

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10.1016/B978-0-12-381320-6.00003-5

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title = "In vivo Analysis of White Adipose Tissue in Zebrafish",

abstract = "White adipose tissue (WAT) is the major site of energy storage in bony vertebrates, and also serves central roles in the endocrine regulation of energy balance. The cellular and molecular mechanisms underlying WAT development and physiology are not well understood. This is due in part to difficulties associated with imaging adipose tissues in mammalian model systems, especially during early life stages. The zebrafish (Danio rerio) has recently emerged as a new model system for adipose tissue research, in which WAT can be imaged in a transparent living vertebrate at all life stages. Here we present detailed methods for labeling adipocytes in live zebrafish using fluorescent lipophilic dyes, and for in vivo microscopy of zebrafish WAT.",

keywords = "Adipocyte, Adipose, Cellularity, Fluorescent, Imaging, Postembryonic",

author = "Minchin, {James E N} and Rawls, {John F.}",

year = "2011",

doi = "10.1016/B978-0-12-381320-6.00003-5",

language = "English",

volume = "105",

pages = "63--86",

journal = "Methods in cell biology",

issn = "0091-679X",

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TY - JOUR

T1 - In vivo Analysis of White Adipose Tissue in Zebrafish

AU - Minchin, James E N

AU - Rawls, John F.

PY - 2011

Y1 - 2011

N2 - White adipose tissue (WAT) is the major site of energy storage in bony vertebrates, and also serves central roles in the endocrine regulation of energy balance. The cellular and molecular mechanisms underlying WAT development and physiology are not well understood. This is due in part to difficulties associated with imaging adipose tissues in mammalian model systems, especially during early life stages. The zebrafish (Danio rerio) has recently emerged as a new model system for adipose tissue research, in which WAT can be imaged in a transparent living vertebrate at all life stages. Here we present detailed methods for labeling adipocytes in live zebrafish using fluorescent lipophilic dyes, and for in vivo microscopy of zebrafish WAT.

AB - White adipose tissue (WAT) is the major site of energy storage in bony vertebrates, and also serves central roles in the endocrine regulation of energy balance. The cellular and molecular mechanisms underlying WAT development and physiology are not well understood. This is due in part to difficulties associated with imaging adipose tissues in mammalian model systems, especially during early life stages. The zebrafish (Danio rerio) has recently emerged as a new model system for adipose tissue research, in which WAT can be imaged in a transparent living vertebrate at all life stages. Here we present detailed methods for labeling adipocytes in live zebrafish using fluorescent lipophilic dyes, and for in vivo microscopy of zebrafish WAT.

KW - Adipocyte

KW - Adipose

KW - Cellularity

KW - Fluorescent

KW - Imaging

KW - Postembryonic

UR - http://www.scopus.com/inward/record.url?scp=80053185109&partnerID=8YFLogxK

U2 - 10.1016/B978-0-12-381320-6.00003-5

DO - 10.1016/B978-0-12-381320-6.00003-5

M3 - Article

C2 - 21951526

AN - SCOPUS:80053185109

SN - 0091-679X

VL - 105

SP - 63

EP - 86

JO - Methods in cell biology

JF - Methods in cell biology

ER -

In vivo Analysis of White Adipose Tissue in Zebrafish

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Keywords / Materials (for Non-textual outputs)

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