In vivo breakdown of dietary [methyl−14C] and [uronate-6−14C]pectin-labelled spinach cell walls by rat intestinal microorganisms and incorporation of 14C into host tissues

C J BUCHANAN, M A EASTWOOD, Stephen Fry

Research output: Contribution to journalArticlepeer-review

Abstract

The metabolism of dietary plant cell wall (PCW) pectin was followed in the rat using PCW isolated from spinach cell cultures C-14-labelled in the galacturonic acid residues or pectic methyl ester groups. C-14-PCW were rapidly broken down in the caecum and colon of the rat and generated several groups of soluble products. [C-14]Methanol was released from [methyl-C-14]pectin-labelled PCW and some of the methanol was converted to C-14-labelled volatile acids. C-14-Labelled volatile acids were also generated from [uronate-6-C-14]pectin-labelled PCW along with soluble but non-volatile material. C-14 derived from both cell wall types was taken up by the caecal and colonic mucosa and transported to the liver, pelt and other body tissues. Some C-14 was excreted as (CO2)-C-14 and in urine.

Original languageEnglish
Pages (from-to)175-185
Number of pages11
JournalJournal of the Science of Food and Agriculture
Volume68
Issue number2
DOIs
Publication statusPublished - Jun 1995

Keywords

  • Plant cell wall
  • pectin
  • fermentation

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