In vivo Degradation and Extracellular Polymer-Binding of Xyloglucan Nonasaccharide, a Naturally-Occurring Anti-Auxin

Elias A.H. Baydoun*, Stephen C. Fry

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

1-10 nM Xyloglucan nonasaccharide (XG9) and its naturally-occurring acylated derivatives (XG9·Ac) antagonise the growth-promoting action of 1μM 2,4-dichlorophenoxyacetic acid in pea stem segments. To investigate the mechanisms involved in controlling the in vivo concentrations of these biologically-active oligosaccharides, we 3H-labelled XG9 and XG9·Ac in their xylose or fucose residues, fed them at 10 nM to rapidly-growing Spinacia cell cultures, and monitored their fate by gel-permeation chromatography. Very little breakdown to low-Mr products took place, and little 3H entered or bound to the cells. However, a proportion of the 3H appeared to become linked to a soluble, extracellular polymer. The linkage was stable to 31% acetic acid and to 0.5M NaOH at 25°C, but Trichoderma cellulase was able to disrupt it, liberating a [3H]oligosaccharide similar to XG9. We speculate that XG9 and XG9·Ac became attached to soluble extracellular xyloglucans by transglycosylation.

Original languageEnglish
Pages (from-to)453-459
Number of pages7
JournalJournal of Plant Physiology
Volume134
Issue number4
DOIs
Publication statusPublished - 1 Jan 1989

Keywords

  • 1,4-(1,3, 1,4)-β-D-glucan 4-glucanohydrolase (E.C. 3.2.1.4)
  • a naturally-occurring acetylated derivative of XG9
  • acetic acid/pyridine/water (1:1:23, v/v/v, pH ca. 4.5)
  • anti-auxin
  • APW
  • auxin
  • BAW
  • BoAW
  • butan-1-ol/acetic acid/water (12:3:5, v/v/v)
  • butanone/acetic acid/water (6:5:5, v/v/v)
  • cell walls
  • cellulase
  • chromatographic mobility relative to that of maltoheptaose
  • EPW
  • ethyl acetate/pyridine/water (8:2:1, v/v/v)
  • gel-permeation chromatography
  • glycosidases
  • GPC
  • k
  • nonasaccharide
  • oligosaccharin
  • paper chromatography
  • PC
  • position of elution during GPC relative to that of dextran (k = 0.00) and glucose (k = 1.00)
  • R
  • Rosa sp. («Paul's Scarlet»)
  • Spinacia oieracea
  • TFA
  • the specific nonasaccharide of xyloglucan illustrated in Fig. 1
  • transglycosylation
  • trifluoroacetic acid
  • XG9
  • XG9·Ac
  • xyloglucan

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