Abstract
The in-vivo formation of a specific nonasaccharide of xyloglucan was investigated. This nonasaccharide has been reported to have biological activity, inhibiting auxin-induced growth in pea stem segments. Cell-suspension cultures of spinach were grown in the presence of [3H]arabinose and [3H]fucose, and the culture-filtrates were examined for oligosaccharides by gelpermeation chromatography and by paper chromatography. Sixteen [3H]pentose-containing oligosaccharides were found, including twelve that contained the sequence [3H]xylosyl-α(1→6)-glucose, which is diagnostic of xyloglucan. In addition, [3H]fucose-containing oligosaccharides of at least three sizes were found. Radiochemical evidence is presented that one of these oligosaccharides was labelled with both [3H]fucose and with [3H]pentose, and was identical with the major xyloglucan-derived nonasaccharide associated with anti-auxin activity. It was largely present in the form of acylated (possibly acetylated) derivatives. It accumulated extracellularly to a steady-state concentration of about 4.3·10-7M. This is the first report of the production of a biologically-active oligosaccharide by living plant cells.
Original language | English |
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Pages (from-to) | 443-453 |
Number of pages | 11 |
Journal | Planta |
Volume | 169 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 Nov 1986 |
Keywords / Materials (for Non-textual outputs)
- "Oligosaccharin"
- Anti-auxin
- Cell wall (active fragment)
- Nonasaccharide
- Spinacia (xyloglucan)
- Xyloglucan