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Abstract / Description of output
Cell fate is established through coordinated gene expression programs in individual cells. Regulatory networks that include the Gata2 transcription factor play central roles in hematopoietic fate establishment. Although Gata2 is essential to the embryonic development and function of hematopoietic stem cells that form the adult hierarchy, little is known about the in vivo expression dynamics of Gata2 in single cells. Here, we examine Gata2 expression in single aortic cells as they establish hematopoietic fate in Gata2Venus mouse embryos. Time-lapse imaging reveals rapid pulsatile level changes in Gata2 reporter expression in cells undergoing endothelial-to-hematopoietic transition. Moreover, Gata2 reporter pulsatile expression is dramatically altered in Gata2+/− aortic cells, which undergo fewer transitions and are reduced in hematopoietic potential. Our novel finding of dynamic pulsatile expression of Gata2 suggests a highly unstable genetic state in single cells concomitant with their transition to hematopoietic fate. This reinforces the notion that threshold levels of Gata2 influence fate establishment and has implications for transcription factor–related hematologic dysfunctions.
Original language | English |
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Pages (from-to) | 233–248 |
Number of pages | 16 |
Journal | Journal of Experimental Medicine |
Volume | 215 |
Issue number | 1 |
Early online date | 7 Dec 2017 |
DOIs | |
Publication status | Published - 2 Jan 2018 |
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Dive into the research topics of 'In vivo single cell analysis reveals Gata2 dynamics in cells transitioning to hematopoietic fate'. Together they form a unique fingerprint.Projects
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Profiles
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Jochen Arlt
- School of Physics and Astronomy - Senior Lecturer
Person: Academic: Research Active (Research Assistant)
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Elaine Dzierzak
- Deanery of Clinical Sciences - Chair of Haematological Regeneration
- Centre for Inflammation Research
- Edinburgh Haematopoiesis Network
Person: Academic: Research Active