In vivo single cell analysis reveals Gata2 dynamics in cells transitioning to hematopoietic fate

Christina Eich, Jochen Arlt, Chris S. Vink, Parham Solaimani Kartalaei, Polynikis Kaimakis, Samanta Mariani, Reinier van der Linden, Wiggert A Van Cappellen, Elaine Dzierzak

Research output: Contribution to journalArticlepeer-review

Abstract

Cell fate is established through coordinated gene expression programs in individual cells. Regulatory networks that include the Gata2 transcription factor play central roles in hematopoietic fate establishment. Although Gata2 is essential to the embryonic development and function of hematopoietic stem cells that form the adult hierarchy, little is known about the in vivo expression dynamics of Gata2 in single cells. Here, we examine Gata2 expression in single aortic cells as they establish hematopoietic fate in Gata2Venus mouse embryos. Time-lapse imaging reveals rapid pulsatile level changes in Gata2 reporter expression in cells undergoing endothelial-to-hematopoietic transition. Moreover, Gata2 reporter pulsatile expression is dramatically altered in Gata2+/− aortic cells, which undergo fewer transitions and are reduced in hematopoietic potential. Our novel finding of dynamic pulsatile expression of Gata2 suggests a highly unstable genetic state in single cells concomitant with their transition to hematopoietic fate. This reinforces the notion that threshold levels of Gata2 influence fate establishment and has implications for transcription factor–related hematologic dysfunctions.
Original languageEnglish
Pages (from-to)233–248
Number of pages16
JournalJournal of Experimental Medicine
Volume215
Issue number1
Early online date7 Dec 2017
DOIs
Publication statusPublished - 2 Jan 2018

Fingerprint Dive into the research topics of 'In vivo single cell analysis reveals Gata2 dynamics in cells transitioning to hematopoietic fate'. Together they form a unique fingerprint.

Cite this