In vivo validation of a miniaturized electrochemical oxygen sensor for measuring intestinal oxygen tension

Mark E. Gray, James R.K. Marland, Camelia Dunare, Ewen O. Blair, James Meehan, Andreas Tsiamis, Ian H. Kunkler, Alan Murray, David Argyle, Alex Dyson, Mervyn Singer, Mark A Potter

Research output: Contribution to journalArticlepeer-review

Abstract

Recent advances in the fields of electronics and microfabrication techniques have led to the development of implantable medical devices for use within the field of precision medicine. Monitoring visceral surface tissue O2 tension (PTo2) by means of an implantable sensor is potentially useful in many clinical situations, including the perioperative management of patients undergoing intestinal resection and anastomosis. This concept could provide a means by which treatment could be tailored to individual patients. This study describes the in vivo validation of a novel, miniaturized electrochemical O2 sensor to provide real-time data on intestinal PTo2. A single O2 sensor was placed onto the serosal surface of the small intestine of anesthetized rats that were exposed to ischemic (superior mesenteric artery occlusion) and hypoxemic (alterations in inspired fractional O2 concentrations) insults. Control experiments demonstrated that the sensors can function and remain stable in an in vivo environment. Intestinal PTo2 decreased following superior mesenteric artery occlusion and with reductions in inspired O2 concentrations. These results were reversible after reinstating blood flow or by increasing inspired O2 concentrations. We have successfully developed an anesthetized rat intestinal ischemic and hypoxic model for validation of a miniaturized O2 sensor to provide real-time measurement of intestinal PTo2. Our results support further validation of the sensors in physiological conditions using a large animal model to provide evidence of their use in clinical applications where monitoring visceral surface tissue O2 tension is important.
Original languageEnglish
Pages (from-to)G242-G252
Number of pages11
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume317
Issue number2
Early online date7 Aug 2019
DOIs
Publication statusE-pub ahead of print - 7 Aug 2019

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