Inactivation of a TSE agent by a novel biorefinement system

R.A. Somerville; K. Fernie; A. Smith; R. Andrews; E. Schmidt; D.M. Taylor

doi:10.1016/j.procbio.2009.06.002

Inactivation of a TSE agent by a novel biorefinement system

R.A. Somerville
, K. Fernie
, A. Smith
, R. Andrews
, E. Schmidt
, D.M. Taylor

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

The infectious agents causing transmissible spongiform encephalopathies (TSEs), sometimes called prions, are notoriously difficult to completely inactivate or destroy. Here we tested a thermal hydrolysis system which combines saturated steam heating to 180 degrees C (10 bar), with stirring. The 301V-TSE strain, which has been derived by passage of BSE in mice, was used since it is the most thermostable TSE strain tested so far. All detectable TSE infectivity was destroyed, with a clearance factor of greater than 10(5) ID50. The use of this technology for the decontamination of TSE infected tissue waste and the potential uses of the end-products are discussed. (C) 2009 Elsevier Ltd. All rights reserved.

Original language	Undefined/Unknown
Pages (from-to)	1060-1062
Number of pages	3
Journal	Process Biochemistry
Volume	44
Issue number	9
DOIs	https://doi.org/10.1016/j.procbio.2009.06.002
Publication status	Published - Sept 2009

Keywords / Materials (for Non-textual outputs)

BSE heat hydrolysis heat inactivation prion TSE

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10.1016/j.procbio.2009.06.002

http://dx.doi.org/10.1016/j.procbio.2009.06.002

Cite this

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title = "Inactivation of a TSE agent by a novel biorefinement system",

abstract = "The infectious agents causing transmissible spongiform encephalopathies (TSEs), sometimes called prions, are notoriously difficult to completely inactivate or destroy. Here we tested a thermal hydrolysis system which combines saturated steam heating to 180 degrees C (10 bar), with stirring. The 301V-TSE strain, which has been derived by passage of BSE in mice, was used since it is the most thermostable TSE strain tested so far. All detectable TSE infectivity was destroyed, with a clearance factor of greater than 10(5) ID50. The use of this technology for the decontamination of TSE infected tissue waste and the potential uses of the end-products are discussed. (C) 2009 Elsevier Ltd. All rights reserved.",

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T1 - Inactivation of a TSE agent by a novel biorefinement system

AU - Somerville, R.A.

AU - Fernie, K.

AU - Smith, A.

AU - Andrews, R.

AU - Schmidt, E.

AU - Taylor, D.M.

N1 - Refereed. Published Prog. 3 Proj. Author allocation: 1;1;1;8;8;7

PY - 2009/9

Y1 - 2009/9

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KW - BSE heat hydrolysis heat inactivation prion TSE

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DO - 10.1016/j.procbio.2009.06.002

M3 - Article

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JO - Process Biochemistry

JF - Process Biochemistry

IS - 9

ER -

Inactivation of a TSE agent by a novel biorefinement system

Abstract

Keywords / Materials (for Non-textual outputs)

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