Induction of early stages of kidney tubule differentiation by lithium ions

Jamie Davies, David Garrod

Research output: Contribution to journalArticlepeer-review

Abstract

Kidney tubules develop by a mesenchyme-epithelium transition, normally induced by ureteric bud through a mechanism that remains obscure. Murine nephrogenesis in vitro has always required heterologous inducing cells. We have discovered that Li+ can elicit the early stages of epithelial differentiation in isolated nephrogenic mesenchyme. We have made detailed comparisons of the timing of morphoregulatory molecule expression between Li+-mediated induction and the traditional in vitro method using induction by spinal cord. Both followed the same program of early morphoregulatory molecule expression, though Li+-induced samples failed to progress into the later parts of the nephrogenic process. Mesenchymes induced by Li+ showed more DNA synthesis than controls, though less than those induced by spinal cord. Discovery of a chemical means to activate differentiation in the absence of heterologous tissue offers a new basis for studying molecular mechanisms regulating the early events of nephrogenesis, as well as for investigating transduction of inductive signals that initiate the process.
Original languageEnglish
Pages (from-to)50–60
JournalDevelopmental Biology
Volume167
Issue number1
DOIs
Publication statusPublished - 1995

Keywords

  • Animals
  • Bucladesine
  • Cell Differentiation
  • DNA
  • Epithelium
  • FEMALE
  • Kidney Tubules
  • Lithium
  • Mesoderm
  • MICE
  • MORPHOGENESIS
  • PREGNANCY
  • Spinal Cord

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