Induction of passive EAE using myelin-reactive CD4+ T cells

Rhoanne C McPherson, Helen E Cambrook, Richard A O'Connor, Stephen M Anderton

Research output: Chapter in Book/Report/Conference proceedingChapter


Experimental autoimmune encephalomyelitis (EAE) is an autoimmune disease of the central nervous system (CNS) often used as a model for the early inflammatory stages of multiple sclerosis and also as a model of organ-specific autoimmune disease.This protocol describes the induction of passive EAE in mice, either using T cells isolated from mice primed with myelin antigens, or through the use of naïve TCR transgenic T cells activated in vitro in the presence of myelin-derived antigens.

Original languageEnglish
Title of host publicationT-Helper Cells
PublisherSpringer New York
Number of pages12
ISBN (Electronic)978-1-4939-1212-4
ISBN (Print)978-1-4939-1211-7
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology


  • Adjuvants, Immunologic
  • Adoptive Transfer
  • Animals
  • CD4-Positive T-Lymphocytes
  • Disease Models, Animal
  • Encephalomyelitis, Autoimmune, Experimental
  • Gene Transfer Techniques
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Mycobacterium tuberculosis
  • Myelin Sheath
  • Receptors, Antigen, T-Cell
  • Th1 Cells

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