Induction of steroid 21-hydroxylase/beta-galactosidase transgene expression by unilateral adrenalectomy

A Lindsay*, A Sharples, SD Morley

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Unilateral adrenalectomy was used to induce compensatory growth in the contralateral adrenal gland of transgenic mice bearing a steroid 21-hydroxylase promoter/beta-galactosidase reporter (21-OHase/beta-gal) transgene, in which 6.4 kb of 5'-flanking sequence of the mouse steroid 21-OHase A gene are linked to a LacZ reporter gene. 48 hours following removal of the right adrenal gland, the left gland of transgene-positive mice showed a 4.5 fold increase in specific activity of the beta-gal reporter, compared to the right gland, while left glands from sham-operated transgene-positive and unilateral adrenalectomized transgene-negative mice showed no such increase. The increased specific transgene reporter activity, relative to total adrenal gland protein, must result from up-regulation of transgene expression, rather than from the compensatory increase in adrenocortical mass. This suggests that elements regulating trophic hormone-mediated 21-OHase gene expression in vivo are located within 6.4 kb of the 21-OHase gene transcription start site.

Original languageEnglish
Pages (from-to)581-585
Number of pages5
JournalEndocrine research
Issue number3-4
Publication statusPublished - 1998
Event8th Conference on the Adrenal Cortex - ORFORD, Canada
Duration: 13 Jun 199816 Jun 1998


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