Infection susceptibility in gastric intrinsic factor (vitamin B12) defective mice is subject to maternal influences

Lynda F Mottram, Anneliese O Speak, Reza M. Selek, Emma L Cambridge, Zoe McIntyre, Leanne Kane, Subhankar Mukhopadhyay, Carolyn Grove, Amy Colin, Maria A. Duque-Corre, Jessica Forbester, Tu Anh Pham Nguyen, Christine Hale, George S. Vasilliou, Mark Arends, Brendan W. Wren, Gordon Dougan, Simon Clare

Research output: Contribution to journalArticlepeer-review

Abstract

Mice harbouring a mutation in the gene encoding gastric intrinsic factor (Gif), a protein essential for the absorption of vitamin B12/cobalamin (Cbl), have potential as a model to explore the role of vitamins in infection. The levels of Cbl in the blood of Gif tm1a/tm1a mutant mice were influenced by the maternal genotype, with offspring born to heterozygous (high Cbl, F1) mothers exhibiting a significantly higher serum Cbl level in comparison to those born to homozygous (low Cbl, F2) equivalents. Low Cbl levels correlated with susceptibility to infectious challenge with Salmonella enterica serovar Typhimurium or Citrobacter rodentium, and this susceptibility phenotype was moderated by Cbl administration. Transcriptional and metabolic profiling revealed Cbl deficient mice exhibited a profile with characteristics of the Warburg effect that was further exacerbated by infection. Our findings demonstrate a role for Cbl in bacterial infection, with potential general relevance to dietary deficiency and infection susceptibility.
Original languageEnglish
Article numbere00830-16
JournalmBio
Volume7
Issue number3
DOIs
Publication statusPublished - 21 Jun 2016

Fingerprint

Dive into the research topics of 'Infection susceptibility in gastric intrinsic factor (vitamin B12) defective mice is subject to maternal influences'. Together they form a unique fingerprint.

Cite this