Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes

Mark P. Ariaans, Peter M. van de Haar, Evert J. Hensen, Lonneke Vervelde*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Infectious Bronchitis Virus, a member of the Coronaviridae, is a respiratory pathogen in poultry. We found that in vitro stimulation with IBV resulted in ChIFN-gamma production in splenocytes of both infected birds and uninfected birds. The non-specific stimulation did not occur when other avian viruses or other coronaviruses were used or when mammalian cells were stimulated with IBV. Inactivation of IBV reduced ChIFN-gamma production, but ChIFN-gamma remained elevated compared to unstimulated cells. An increase in ChIFN-gamma mRNA was detected in splenocytes from IBV-infected and uninfected chickens as early as 1 h after stimulation with IBV. These results indicate that IBV acts as a polyclonal stimulus, inducing a rapid production of IFN-gamma even without previous exposure to the virus. (c) 2008 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)68-73
Number of pages6
JournalVirology
Volume385
Issue number1
DOIs
Publication statusPublished - 1 Mar 2009

Keywords

  • SARS-COV
  • Interferon-gamma
  • PROTEIN
  • INDUCTION
  • Chicken
  • IFN-GAMMA
  • Infectious Bronchitis Virus
  • VACCINE DEVELOPMENT
  • Polyclonal stimulation
  • CYTOKINE RESPONSES
  • Superantigen
  • DISEASE
  • CORONAVIRUS
  • ELISPOT
  • ACUTE RESPIRATORY SYNDROME
  • REPLICATION

Fingerprint

Dive into the research topics of 'Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes'. Together they form a unique fingerprint.

Cite this