Inflammatory cytokine responses in a pregnant mouse model of Chlamydophila abortus infection

Karen Kerr, Nicholas Wheelhouse, Morag Livingstone, Ian E Anderson, Gary Entrican, Declan McKeever, David Longbottom

Research output: Contribution to journalArticlepeer-review

Abstract

Chlamydophila abortus (C. abortus) is the aetiological agent of ovine enzootic abortion (OEA). The highly elevated expression of the pro-inflammatory cytokine tumour necrosis factor-alpha (TNFalpha) and low-level expression of interferon-gamma (IFNgamma) that are detected in C. abortus-infected placentas have been implicated in the pathogenesis of OEA. Late-term abortions similar to those occurring in sheep have also been observed in mouse models of C. abortus infection. Since mouse studies have contributed significantly to our understanding of the immunological responses to chlamydial infections and serve as a good model for rapidly assessing candidate vaccines for OEA, we investigated local expression of TNFalpha and IFNgamma in infected mice. At various time points over the course of infection mice were sacrificed, serum samples obtained for serum antibody and cytokine analyses, and livers and placental tissues were removed and fixed to determine C. abortus colonisation and cytokine expression. Immunostaining for C. abortus was significantly greater in placenta compared to liver (P
Original languageEnglish
Pages (from-to)392-8
Number of pages7
JournalVeterinary Microbiology
Volume144
Issue number3-4
DOIs
Publication statusPublished - 26 Aug 2010

Keywords

  • Animals
  • Antigens, Bacterial
  • Chlamydophila
  • Chlamydophila Infections
  • Cytokines
  • Female
  • Gene Expression Regulation
  • Interferon-gamma
  • Liver
  • Mice
  • Placenta
  • Pregnancy
  • RNA, Messenger
  • Tissue Distribution
  • Tumor Necrosis Factor-alpha

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