Influence of Base Dynamics on the Conformational Properties of DNA:  Observation of Static Conformational States in Rigid Duplexes at 77 K

Robert K. Neely, Anita C. Jones

Research output: Contribution to journalArticlepeer-review

Abstract

Time-resolved fluorescence of 2-aminopurine-labeled DNA duplexes at 77 K reveals the relationship between base dynamics and the conformational heterogeneity that results in the well-known multiexponential fluorescence decay at room temperature. The conformation that exhibits rapid interbase charge transfer at room temperature is not populated in the frozen duplex at 77 K; this geometry is accessed by thermal motion of the bases, it is not a minimum energy structure of the duplex. Three photophysically distinct conformational states persist in the frozen duplex; these are minimum energy structures and do not interconvert at room temperature on the time scale of the 2-aminopurine excited-state lifetime.
Original languageEnglish
Pages (from-to)15952-15953
JournalJournal of the American Chemical Society
Volume128
Issue number50
DOIs
Publication statusPublished - 1 Dec 2006

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