Inhibition of infectious bursal disease virus infection by artificial microRNAs targeting chicken heat-shock protein 90

Weifeng Yuan, Xinyu Zhang, Xiaoli Xia, Huaichang Sun

Research output: Contribution to journalArticlepeer-review

Abstract

Infectious bursal disease virus (IBDV) causes an important disease in young chickens. Chicken heat-shock protein 90 (cHsp90) has been shown to be a functional component of the cellular receptor complex for IBDV infection. This study demonstrates the inhibitory effect of vector-expressed anti-cHsp90α microRNA (miRNA) on IBDV infection. The reporter vectors pcHsp90α-EGFP and pcHsp90β-EGFP were constructed to facilitate effective miRNA selection. Two anti-cHsp90α and one anti-cHsp90β miRNA-expression vectors were constructed for a stable transfection study. Poly(A)-tailed RT-PCR detected sequence-specific miRNA transcription in transfected cells. Semiquantitative RT-PCR showed inhibition of cHsp90 transcription in transfected cells. A virus-titration assay showed that the anti-cHsp90α miRNA, but not the anti-cHsp90β miRNA, had inhibitory effects on IBDV infection. These results suggest that cHsp90α is a functional component of the cellular receptor complex for IBDV infection, and that anti-cHsp90α miRNA could be used as an anti-IBDV reagent.
Original languageEnglish
Pages (from-to)876-9
Number of pages4
JournalJournal of General Virology
Volume93
Issue numberPt 4
DOIs
Publication statusPublished - Apr 2012

Keywords

  • Animals
  • Birnaviridae Infections
  • Cell Line
  • Chickens
  • HSP90 Heat-Shock Proteins
  • Infectious bursal disease virus
  • MicroRNAs
  • Polymerase Chain Reaction
  • Poultry Diseases
  • Receptors, Virus
  • Transcription, Genetic
  • Transfection

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