Inhibition of influenza virus replication by -amanitin: mode of action

S J Armstrong, N D Hastie, B W Mahy

Research output: Contribution to journalArticlepeer-review

Abstract

The replication of influenza virus in chick embryo fibroblast cells is inhibited by alpha-amanitin added during the first 2 hr of infection at concentrations similar to those required to inhibit cellular DNA-dependent RNA polymerase form II in vivo. Of two periods of increased RNA synthesis observed in cells infected with influenza virus, only the first, occurring from 0 to 2 hr after infection, is sensitive to alpha-amanitin. During this early period, there is a stimulation of the activity of DNA-dependent RNA polymerase II of nuclei isolated from infected cells. The data suggest that DNA transcription mediated by polymerase II is essential for influenza virus replication.

Original languageEnglish
Pages (from-to)1421-4
Number of pages4
JournalProceedings of the National Academy of Sciences
Volume69
Issue number6
Publication statusPublished - Jun 1972

Keywords

  • Animals
  • Antiviral Agents
  • Cells, Cultured
  • Chick Embryo
  • DNA
  • Depression, Chemical
  • Fibroblasts
  • Mycotoxins
  • Orthomyxoviridae
  • Peptides
  • RNA
  • RNA Nucleotidyltransferases
  • Virus Replication

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