Inhibition of p53-dependent transcription by BOX-I phospho-peptide mimetics that bind to p300

D Dornan, T R Hupp

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The N-terminal BOX-I domain of p53 containing a docking site for the negative regulator MDM2 and the positive effector p300, harbours two recently identified phosphorylation sites at Thr18 or Ser20O whose affect on p300 is undefined. Biochemical assays demonstrate that although MDM2 binding is inhibited by these phosphorylations, p300 binding is strikingly stabilized by Thr18 or Ser20 phosphorylation. Introducing EGFP-BOX-I domain peptides with an aspartate substitution at Thr18 or Ser20 induced a significant inhibition of endogenous p53-dependent transcription in cycling cells, in irradiated cells, as well as in cells transiently co-transfected with p300 and p53. In contrast an EGFP-wild-type BOX-I domain peptide stimulated p53 activity via inhibition of MDM2 protein binding. These results suggest that phosphorylation of p53 at Thr18 or Ser20 can activate p53 by stabilizing the p300-p53 complex and also identify a class of small molecular weight ligands capable of selective discrimination between MDM2- and p300-dependent activities.
Original languageEnglish
Pages (from-to)139-44
Number of pages6
JournalEMBO Reports
Issue number2
Publication statusPublished - Feb 2001

Keywords / Materials (for Non-textual outputs)

  • Amino Acid Substitution
  • Binding Sites
  • Humans
  • Ligands
  • Molecular Mimicry
  • Mutation
  • Nuclear Proteins
  • Phosphopeptides
  • Phosphorylation
  • Phosphoserine
  • Phosphothreonine
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-mdm2
  • Trans-Activators
  • Transcription, Genetic
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53


Dive into the research topics of 'Inhibition of p53-dependent transcription by BOX-I phospho-peptide mimetics that bind to p300'. Together they form a unique fingerprint.

Cite this