Abstract
Recently, we showed a natural reprogramming process during infection with Mycobacterium leprae (ML), the causative organism of human leprosy. ML hijacks the notable plasticity of adult Schwann cells in the peripheral nervous system (PNS), bacteria's preferred nonimmune niche, to reprogram infected cells to progenitor/stem cell–like cells (pSLCs). Whereas ML appear to use this reprogramming process as a sophisticated bacterial strategy to spread infection to other tissues, understanding the mechanisms may shed new insights into the basic biology of cellular reprogramming and the development of new approaches for generating pSLC for therapeutic purposes as well as targeting bacterial infectious diseases at an early stage. Toward these goals, we extended our studies to identify other players that might be involved in this complex host cell reprogramming. Here we show that ML activates numerous immune-related genes mainly involved in innate immune responses and inflammation during early infection before downregulating Schwann cell lineage genes and reactivating developmental transcription factors. We validated these findings by demonstrating the ability of infected cells to secrete soluble immune factor proteins at early time points and their continued release during the course of reprogramming. By using time-lapse microscopy and a migration assay with reprogrammed Schwann cells (pSLCs) cultured with macrophages, we show that reprogrammed cells possess the ability to attract macrophages, providing evidence for a functional role of immune gene products during reprogramming. These findings suggest a potential role of innate immune response and the related signaling pathways in cellular reprogramming and the initiation of neuropathogenesis during ML infection.
Original language | English |
---|---|
Pages (from-to) | 9-17 |
Journal | Cellular Reprogramming |
Volume | 16 |
Issue number | 1 |
Early online date | 26 Nov 2013 |
DOIs | |
Publication status | Published - Feb 2014 |
Fingerprint
Dive into the research topics of 'Innate Immune Response Precedes Mycobacterium leprae–Induced Reprogramming of Adult Schwann Cells'. Together they form a unique fingerprint.Profiles
-
Anura Rambukkana
- Deanery of Biomedical Sciences - Chair of Regeneration Biology
- Centre for Regenerative Medicine
- Centre for Discovery Brain Sciences
- Edinburgh Neuroscience
Person: Academic: Research Active