Innate immunity: Bacterial cell-wall muramyl peptide targets the conserved transcription factor YB-1

A. G. Laman; R. Lathe; G. V. Savinov; A. O. Shepelyakovskaya; Kh M. Boziev; L. K. Baidakova; A. N. Chulin; F. A. Brovko; E. V. Svirshchevskaya; Y. Kotelevtsev; I. A. Eliseeva; S. G. Guryanov; D. N. Lyabin; L. P. Ovchinnikov; V. T. Ivanov

doi:10.1016/j.febslet.2015.05.028

Innate immunity: Bacterial cell-wall muramyl peptide targets the conserved transcription factor YB-1

A. G. Laman^*, R. Lathe, G. V. Savinov, A. O. Shepelyakovskaya, Kh M. Boziev, L. K. Baidakova, A. N. Chulin, F. A. Brovko, E. V. Svirshchevskaya, Y. Kotelevtsev, I. A. Eliseeva, S. G. Guryanov, D. N. Lyabin, L. P. Ovchinnikov, V. T. Ivanov

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Abstract The bacterial cell wall muramyl dipeptides MDP and glucosaminyl-MDP (GMDP) are powerful immunostimulators but their binding target remains controversial. We previously reported expression cloning of GMDP-binding polypeptides and identification of Y-box protein 1 (YB-1) as their sole target. Here we show specific binding of GMDP to recombinant YB-1 protein and subcellular colocalization of YB-1 and GMDP. GMDP binding to YB-1 upregulated gene expression levels of NF-κB2, a mediator of innate immunity. Furthermore, YB-1 knockdown abolished GMDP-induced Nfkb2 expression. GMDP/YB-1 stimulation led to NF-κB2 cleavage, transport of activated NF-κB2 p52 to the nucleus, and upregulation of NF-κB2-dependent chemokine Cxcr4 gene expression. Therefore, our findings identify YB-1 as new target for muramyl peptide signaling.

Original language	English
Article number	37184
Pages (from-to)	1819-1824
Number of pages	6
Journal	FEBS Letters
Volume	589
Issue number	15
DOIs	https://doi.org/10.1016/j.febslet.2015.05.028
Publication status	Published - 3 Jul 2015

Keywords / Materials (for Non-textual outputs)

Glucosaminyl-muramyl dipeptide
Innate immunity
Muramyl peptide
Y-box protein 1

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10.1016/j.febslet.2015.05.028

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Laman, A. G., Lathe, R., Savinov, G. V., Shepelyakovskaya, A. O., Boziev, K. M., Baidakova, L. K., Chulin, A. N., Brovko, F. A., Svirshchevskaya, E. V., Kotelevtsev, Y., Eliseeva, I. A., Guryanov, S. G., Lyabin, D. N., Ovchinnikov, L. P., & Ivanov, V. T. (2015). Innate immunity: Bacterial cell-wall muramyl peptide targets the conserved transcription factor YB-1. FEBS Letters, 589(15), 1819-1824. Article 37184. https://doi.org/10.1016/j.febslet.2015.05.028

@article{0a1fc02644214ec48d948c12c44038ea,

title = "Innate immunity: Bacterial cell-wall muramyl peptide targets the conserved transcription factor YB-1",

abstract = "Abstract The bacterial cell wall muramyl dipeptides MDP and glucosaminyl-MDP (GMDP) are powerful immunostimulators but their binding target remains controversial. We previously reported expression cloning of GMDP-binding polypeptides and identification of Y-box protein 1 (YB-1) as their sole target. Here we show specific binding of GMDP to recombinant YB-1 protein and subcellular colocalization of YB-1 and GMDP. GMDP binding to YB-1 upregulated gene expression levels of NF-κB2, a mediator of innate immunity. Furthermore, YB-1 knockdown abolished GMDP-induced Nfkb2 expression. GMDP/YB-1 stimulation led to NF-κB2 cleavage, transport of activated NF-κB2 p52 to the nucleus, and upregulation of NF-κB2-dependent chemokine Cxcr4 gene expression. Therefore, our findings identify YB-1 as new target for muramyl peptide signaling.",

keywords = "Glucosaminyl-muramyl dipeptide, Innate immunity, Muramyl peptide, Y-box protein 1",

author = "Laman, {A. G.} and R. Lathe and Savinov, {G. V.} and Shepelyakovskaya, {A. O.} and Boziev, {Kh M.} and Baidakova, {L. K.} and Chulin, {A. N.} and Brovko, {F. A.} and Svirshchevskaya, {E. V.} and Y. Kotelevtsev and Eliseeva, {I. A.} and Guryanov, {S. G.} and Lyabin, {D. N.} and Ovchinnikov, {L. P.} and Ivanov, {V. T.}",

year = "2015",

month = jul,

day = "3",

doi = "10.1016/j.febslet.2015.05.028",

language = "English",

volume = "589",

pages = "1819--1824",

journal = "FEBS Letters",

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Laman, AG, Lathe, R, Savinov, GV, Shepelyakovskaya, AO, Boziev, KM, Baidakova, LK, Chulin, AN, Brovko, FA, Svirshchevskaya, EV, Kotelevtsev, Y, Eliseeva, IA, Guryanov, SG, Lyabin, DN, Ovchinnikov, LP & Ivanov, VT 2015, 'Innate immunity: Bacterial cell-wall muramyl peptide targets the conserved transcription factor YB-1', FEBS Letters, vol. 589, no. 15, 37184, pp. 1819-1824. https://doi.org/10.1016/j.febslet.2015.05.028

TY - JOUR

T1 - Innate immunity

T2 - Bacterial cell-wall muramyl peptide targets the conserved transcription factor YB-1

AU - Laman, A. G.

AU - Lathe, R.

AU - Savinov, G. V.

AU - Shepelyakovskaya, A. O.

AU - Boziev, Kh M.

AU - Baidakova, L. K.

AU - Chulin, A. N.

AU - Brovko, F. A.

AU - Svirshchevskaya, E. V.

AU - Kotelevtsev, Y.

AU - Eliseeva, I. A.

AU - Guryanov, S. G.

AU - Lyabin, D. N.

AU - Ovchinnikov, L. P.

AU - Ivanov, V. T.

PY - 2015/7/3

Y1 - 2015/7/3

N2 - Abstract The bacterial cell wall muramyl dipeptides MDP and glucosaminyl-MDP (GMDP) are powerful immunostimulators but their binding target remains controversial. We previously reported expression cloning of GMDP-binding polypeptides and identification of Y-box protein 1 (YB-1) as their sole target. Here we show specific binding of GMDP to recombinant YB-1 protein and subcellular colocalization of YB-1 and GMDP. GMDP binding to YB-1 upregulated gene expression levels of NF-κB2, a mediator of innate immunity. Furthermore, YB-1 knockdown abolished GMDP-induced Nfkb2 expression. GMDP/YB-1 stimulation led to NF-κB2 cleavage, transport of activated NF-κB2 p52 to the nucleus, and upregulation of NF-κB2-dependent chemokine Cxcr4 gene expression. Therefore, our findings identify YB-1 as new target for muramyl peptide signaling.

AB - Abstract The bacterial cell wall muramyl dipeptides MDP and glucosaminyl-MDP (GMDP) are powerful immunostimulators but their binding target remains controversial. We previously reported expression cloning of GMDP-binding polypeptides and identification of Y-box protein 1 (YB-1) as their sole target. Here we show specific binding of GMDP to recombinant YB-1 protein and subcellular colocalization of YB-1 and GMDP. GMDP binding to YB-1 upregulated gene expression levels of NF-κB2, a mediator of innate immunity. Furthermore, YB-1 knockdown abolished GMDP-induced Nfkb2 expression. GMDP/YB-1 stimulation led to NF-κB2 cleavage, transport of activated NF-κB2 p52 to the nucleus, and upregulation of NF-κB2-dependent chemokine Cxcr4 gene expression. Therefore, our findings identify YB-1 as new target for muramyl peptide signaling.

KW - Glucosaminyl-muramyl dipeptide

KW - Innate immunity

KW - Muramyl peptide

KW - Y-box protein 1

UR - http://www.scopus.com/inward/record.url?scp=84934435116&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2015.05.028

DO - 10.1016/j.febslet.2015.05.028

M3 - Article

C2 - 26026270

AN - SCOPUS:84934435116

SN - 0014-5793

VL - 589

SP - 1819

EP - 1824

JO - FEBS Letters

JF - FEBS Letters

IS - 15

M1 - 37184

ER -

Innate immunity: Bacterial cell-wall muramyl peptide targets the conserved transcription factor YB-1

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