Inositol 1,4,5-trisphosphate receptor and dSTIM function in Drosophila insulin-producing neurons regulates systemic intracellular calcium homeostasis and flight

Neha Agrawal, Gayatri Venkiteswaran, Sufia Sadaf, Nisha Padmanabhan, Santanu Banerjee, Gaiti Hasan

Research output: Contribution to journalArticlepeer-review

Abstract

Calcium (Ca(2+)) signaling is known to regulate the development, maintenance and modulation of activity in neuronal circuits that underlie organismal behavior. In Drosophila, intracellular Ca(2+) signaling by the inositol 1,4,5-trisphosphate receptor and the store-operated channel (dOrai) regulates the formation and function of neuronal circuits that control flight. Here, we show that restoring InsP(3)R activity in insulin-producing neurons of flightless InsP(3)R mutants (itpr) during pupal development can rescue systemic flight ability. Expression of the store operated Ca(2+) entry (SOCE) regulator dSTIM in insulin-producing neurons also suppresses compromised flight ability of InsP(3)R mutants suggesting that SOCE can compensate for impaired InsP(3)R function. Despite restricted expression of wild-type InsP(3)R and dSTIM in insulin-producing neurons, a global restoration of SOCE and store Ca(2+) is observed in primary neuronal cultures from the itpr mutant. These results suggest that restoring InsP(3)R-mediated Ca(2+) release and SOCE in a limited subset of neuromodulatory cells can influence systemic behaviors such as flight by regulating intracellular Ca(2+) homeostasis in a large population of neurons through a non-cell-autonomous mechanism.

Original languageEnglish
Pages (from-to)1301-13
Number of pages13
JournalJournal of Neuroscience
Volume30
Issue number4
DOIs
Publication statusPublished - 27 Jan 2010

Keywords

  • Animals
  • Calcium/metabolism
  • Calcium Signaling/physiology
  • Cell Membrane/genetics
  • Cells, Cultured
  • Central Nervous System/cytology
  • Drosophila
  • Drosophila Proteins/genetics
  • Flight, Animal/physiology
  • Homeostasis/physiology
  • Inositol 1,4,5-Trisphosphate Receptors/genetics
  • Insulin/metabolism
  • Insulin Secretion
  • Intracellular Fluid/metabolism
  • Membrane Proteins/genetics
  • Mutation/genetics
  • Neural Pathways/cytology
  • Neurons/cytology
  • Pupa/genetics
  • Stromal Interaction Molecule 1

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