Installation of an organocatalyst into a protein scaffold creates an artificial Stetterase

Alice MacAulay, Eva Klemencic, Richard C. Brewster, Süleyman Mert Ünal, Evangelia Notari, Christopher W. Wood, Amanda G. Jarvis*, Dominic J. Campopiano*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Using a protein scaffold covalently functionalised with a thiamine-inspired N-heterocyclic carbene (NHC), we created an artificial Stetterase (ArtiSt) which catalyses a stereoselective, intramolecular Stetter reaction. We demonstrate that ArtiSt functions under ambient conditions with low catalyst loading. Furthermore, activity can be increased >20 fold by altering the protein scaffold.

Original languageEnglish
Pages (from-to)13746-13749
JournalChemical Communications
Volume60
Issue number93
Early online date25 Oct 2024
DOIs
Publication statusE-pub ahead of print - 25 Oct 2024

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