Integrated Top-Down/Bottom-Up Mass Spectrometry of Proteins Using a Droplet Microfluidic Platform

Adam Stokes, Logan Mackay, Daniel Gruber, Yifan Li, David Clarke, Anthony Walton, Pat Langridge-Smith

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have used droplet microfluidic (DMF) platform as front-end sample preparation technique prior to ESI massspectrometric
analysis of protein samples. DMF is a micro-electromechanical system (MEMS) technology capable of moving,
splitting, merging and dispensing of sub-microlitre droplets. We report on integration of DMF with ESI-MS using a liquid
handling robot and demonstrate sample enrichment using functionalized magnetic beads to perform on-chip enrichment
of a HIS-tagged protein from a clarified cell lysate droplet, followed by alternatively a bottom-up or top-down proteomics
mass-spectrometric workflow. This demonstrates the utility of DMF for low-volume automated sample handling for mass
spectrometric analysis.
Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Pages1644
Number of pages3
Publication statusPublished - 2 Oct 2011

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