TY - JOUR
T1 - Interaction between the nasal microbiota and S. pneumoniae in the context of live-attenuated influenza vaccine
AU - De Steenhuijsen Piters, Wouter A. A.
AU - Jochems, Simon P.
AU - Mitsi, Elena
AU - Rylance, Jamie
AU - Pojar, Sherin
AU - Nikolaou, Elissavet
AU - German, Esther L.
AU - Holloway, Mark
AU - Carniel, Beatriz F.
AU - Chu, Mei Ling J. N.
AU - Arp, Kayleigh
AU - Sanders, Elisabeth A. M.
AU - Ferreira, Daniela M.
AU - Bogaert, Debby
PY - 2019/7/5
Y1 - 2019/7/5
N2 - S. pneumoniae is the main bacterial pathogen involved in pneumonia. Pneumococcal acquisition and colonization density is likely affected by viral co-infections, the local microbiome composition and mucosal immunity. Here, we report the interactions between live-attenuated influenza vaccine (LAIV), successive pneumococcal challenge, and the healthy adult nasal microbiota and mucosal immunity using a human experimental challenge model. Nasal microbiota profiles at baseline are associated with consecutive pneumococcal carriage outcome (non-carrier, low-dense and high-dense pneumococcal carrier), independent of LAIV co-administration. Corynebacterium/Dolosigranulum-dominated profiles are associated with low-density colonization. Lowest rates of natural viral co-infection at baseline and post-LAIV influenza replication are detected in the low-density carriers. Also, we observed the fewest microbiota perturbations and mucosal cytokine responses in the low-density carriers compared to non-carriers or high-density carriers. These results indicate that the complete respiratory ecosystem affects pneumococcal behaviour following challenge, with low-density carriage representing the most stable ecological state.
AB - S. pneumoniae is the main bacterial pathogen involved in pneumonia. Pneumococcal acquisition and colonization density is likely affected by viral co-infections, the local microbiome composition and mucosal immunity. Here, we report the interactions between live-attenuated influenza vaccine (LAIV), successive pneumococcal challenge, and the healthy adult nasal microbiota and mucosal immunity using a human experimental challenge model. Nasal microbiota profiles at baseline are associated with consecutive pneumococcal carriage outcome (non-carrier, low-dense and high-dense pneumococcal carrier), independent of LAIV co-administration. Corynebacterium/Dolosigranulum-dominated profiles are associated with low-density colonization. Lowest rates of natural viral co-infection at baseline and post-LAIV influenza replication are detected in the low-density carriers. Also, we observed the fewest microbiota perturbations and mucosal cytokine responses in the low-density carriers compared to non-carriers or high-density carriers. These results indicate that the complete respiratory ecosystem affects pneumococcal behaviour following challenge, with low-density carriage representing the most stable ecological state.
U2 - 10.1038/s41467-019-10814-9
DO - 10.1038/s41467-019-10814-9
M3 - Article
SN - 2041-1723
VL - 10
JO - Nature Communications
JF - Nature Communications
IS - 1
ER -