Interactions among mitochondrial proteins altered in glioblastoma

Ruth F Deighton*, Thierry Le Bihan, Sarah Martin, Alice M J Gerth, Mailis McCulloch, Julia M Edgar, Lorraine E Kerr, Ian R Whittle, James McCulloch

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Mitochondrial dysfunction is putatively central to glioblastoma (GBM) pathophysiology but there has been no systematic analysis in GBM of the proteins which are integral to mitochondrial function. Alterations in proteins in mitochondrial enriched fractions from patients with GBM were defined with label-free liquid chromatography mass spectrometry. 256 mitochondrially-associated proteins were identified in mitochondrial enriched fractions and 117 of these mitochondrial proteins were markedly (fold-change ≥2) and significantly altered in GBM (p ≤ 0.05). Proteins associated with oxidative damage (including catalase, superoxide dismutase 2, peroxiredoxin 1 and peroxiredoxin 4) were increased in GBM. Protein-protein interaction analysis highlighted a reduction in multiple proteins coupled to energy metabolism (in particular respiratory chain proteins, including 23 complex-I proteins). Qualitative ultrastructural analysis in GBM with electron microscopy showed a notably higher prevalence of mitochondria with cristolysis in GBM. This study highlights the complex mitochondrial proteomic adjustments which occur in GBM pathophysiology.

Original languageEnglish
Pages (from-to)247-256
Number of pages10
JournalJournal of Neuro-Oncology
Volume118
Issue number2
DOIs
Publication statusPublished - 1 Jan 2014

Keywords

  • Clinical proteomics
  • Glioblastoma
  • Mitochondria

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