Interactions between cellular regulatory proteins and a unique sequence region in the human cytomegalovirus major immediate-early promoter

P Ghazal, H Lubon, C Reynolds-Kohler, L Hennighausen, J A Nelson

Research output: Contribution to journalArticlepeer-review

Abstract

Transcription from the human cytomegalovirus major immediate-early promoter is dependent on host-cell regulatory proteins. The interactions between cellular nuclear proteins and a unique sequence located from nucleotide position -660 to -540 was investigated. The unique region presents a defined target for multiple distinct DNA-binding proteins which appear, in part, to have overlapping binding sites. A minimum of five sequence-specific DNA-binding activities that interact with sequences between -632 and -602, -602 and -557, -602 and -590, -563 and -540, and -602 and -582 were detected. Evidence is presented to suggest that the -632 to -602 site, a previously characterized nuclear factor 1 binding site, does not bind NF1 but strongly interacts with a distinct cellular factor. The binding of cellular proteins to the unique sequence region was shown to be important in directing transcription from the major immediate-early promoter.
Original languageEnglish
Pages (from-to)18-25
Number of pages8
JournalJournal of Virology
Volume174
Issue number1
Publication statusPublished - 1990

Keywords

  • Antigens, Viral
  • Base Sequence
  • Binding Sites
  • Chromatography
  • Cytomegalovirus
  • DNA, Viral
  • DNA-Binding Proteins
  • HeLa Cells
  • Humans
  • Immediate-Early Proteins
  • Molecular Sequence Data
  • Nuclear Proteins
  • Promoter Regions, Genetic
  • Transcription Factors
  • Transcription, Genetic
  • Transfection

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