Interactions of the site-specific recombinases XerC and XerD with the recombination site dif

G W Blakely, D J Sherratt

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The Xer site-specific recombination system of Escherichia coli is involved in the stable inheritance of circular replicons. Multimeric replicons, produced by homologous recombination, are converted to monomers by the action of two related recombinases XerC and XerD. Site-specific recombination at a locus, dif, within the chromosomal replication terminus region is thought to convert dimeric chromosomes to monomers, which can then be segregated prior to cell division. The recombinases XerC and XerD bind cooperatively to dif, where they catalyse recombination. Chemical modification of specific bases and the phosphate-sugar backbone within dif was used to investigate the requirements for binding of the recombinases. Site-directed mutagenesis was then used to alter bases implicated in recombinase binding. Characterization of these mutants by in vitro recombinase binding and in vivo recombination, has demonstrated that the cooperative interactions between XerC and XerD can partially overcome DNA alterations that should interfere with specific recombinase-dif interactions.
Original languageEnglish
Pages (from-to)5613-20
Number of pages8
JournalNucleic Acids Research
Volume22
Issue number25
DOIs
Publication statusPublished - 1994

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