Interferon-γ expression in trophoblast cells in pregnant ewes challenged with Chlamydophila abortus

S. Worrall; D. J. Sammin; H. F. Bassett; C. R. Reid; J. Gutierrez; P. X. Marques; J. E. Nally; J. O'Donovan; E. J. Williams; A. Proctor; B. K. Markey

doi:10.1016/j.jri.2011.03.011

Interferon-γ expression in trophoblast cells in pregnant ewes challenged with Chlamydophila abortus

S. Worrall^*, D. J. Sammin, H. F. Bassett, C. R. Reid, J. Gutierrez, P. X. Marques, J. E. Nally, J. O'Donovan, E. J. Williams, A. Proctor, B. K. Markey

^*Corresponding author for this work

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Pregnant ewes were challenged with Chlamydia abortus at 91-98 days of gestation and euthanised at 14, 21 and 28 days post-challenge. IFNγ mRNA labelling appeared to be co-localised with Chlamydial lipopolysaccharide within trophoblast cells in discrete areas lining the primary villi in the limbus and hilar zone of the placentomes from challenged sheep on days 21 and 28 post-infection. The presence of IFNγ was also demonstrated by immunohistochemistry. No labelling was seen in tissues from the non-infected ewes. The presence of IFNγ in trophoblast cells from infected ewes may indicate an attempt to restrict the replication of the organism and be an important trigger for the inflammatory responses that develop on the fetal side of the placenta in enzootic abortion.

Original language	English
Pages (from-to)	214-219
Number of pages	6
Journal	Journal of Reproductive Immunology
Volume	90
Issue number	2
DOIs	https://doi.org/10.1016/j.jri.2011.03.011
Publication status	Published - Aug 2011

Keywords / Materials (for Non-textual outputs)

Chlamydophila abortus
In situ hybridization
Interferon-gamma
Ovine enzootic abortion
Trophoblast cells

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10.1016/j.jri.2011.03.011

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@article{054ed3fdbc9144e08b26026855a5dcf2,

title = "Interferon-γ expression in trophoblast cells in pregnant ewes challenged with Chlamydophila abortus",

abstract = "Pregnant ewes were challenged with Chlamydia abortus at 91-98 days of gestation and euthanised at 14, 21 and 28 days post-challenge. IFNγ mRNA labelling appeared to be co-localised with Chlamydial lipopolysaccharide within trophoblast cells in discrete areas lining the primary villi in the limbus and hilar zone of the placentomes from challenged sheep on days 21 and 28 post-infection. The presence of IFNγ was also demonstrated by immunohistochemistry. No labelling was seen in tissues from the non-infected ewes. The presence of IFNγ in trophoblast cells from infected ewes may indicate an attempt to restrict the replication of the organism and be an important trigger for the inflammatory responses that develop on the fetal side of the placenta in enzootic abortion.",

keywords = "Chlamydophila abortus, In situ hybridization, Interferon-gamma, Ovine enzootic abortion, Trophoblast cells",

author = "S. Worrall and Sammin, {D. J.} and Bassett, {H. F.} and Reid, {C. R.} and J. Gutierrez and Marques, {P. X.} and Nally, {J. E.} and J. O'Donovan and Williams, {E. J.} and A. Proctor and Markey, {B. K.}",

note = "Funding Information: This work was supported by funding from the Research Stimulus Fund of the Irish Department of Agriculture, Fisheries and Food . We also acknowledge the support of COST (European Cooperation in Science and Technology). Frank Malone, Department of Agriculture and Rural Development for Northern Ireland, is thanked for providing the ovine M. bovis-infected lymph node blocks. The authors also thank Brian Cloak for photographic work and Natasha Nally for expert technical assistance.",

year = "2011",

month = aug,

doi = "10.1016/j.jri.2011.03.011",

language = "English",

volume = "90",

pages = "214--219",

journal = "Journal of Reproductive Immunology",

issn = "0165-0378",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Interferon-γ expression in trophoblast cells in pregnant ewes challenged with Chlamydophila abortus

AU - Worrall, S.

AU - Sammin, D. J.

AU - Bassett, H. F.

AU - Reid, C. R.

AU - Gutierrez, J.

AU - Marques, P. X.

AU - Nally, J. E.

AU - O'Donovan, J.

AU - Williams, E. J.

AU - Proctor, A.

AU - Markey, B. K.

N1 - Funding Information: This work was supported by funding from the Research Stimulus Fund of the Irish Department of Agriculture, Fisheries and Food . We also acknowledge the support of COST (European Cooperation in Science and Technology). Frank Malone, Department of Agriculture and Rural Development for Northern Ireland, is thanked for providing the ovine M. bovis-infected lymph node blocks. The authors also thank Brian Cloak for photographic work and Natasha Nally for expert technical assistance.

PY - 2011/8

Y1 - 2011/8

N2 - Pregnant ewes were challenged with Chlamydia abortus at 91-98 days of gestation and euthanised at 14, 21 and 28 days post-challenge. IFNγ mRNA labelling appeared to be co-localised with Chlamydial lipopolysaccharide within trophoblast cells in discrete areas lining the primary villi in the limbus and hilar zone of the placentomes from challenged sheep on days 21 and 28 post-infection. The presence of IFNγ was also demonstrated by immunohistochemistry. No labelling was seen in tissues from the non-infected ewes. The presence of IFNγ in trophoblast cells from infected ewes may indicate an attempt to restrict the replication of the organism and be an important trigger for the inflammatory responses that develop on the fetal side of the placenta in enzootic abortion.

AB - Pregnant ewes were challenged with Chlamydia abortus at 91-98 days of gestation and euthanised at 14, 21 and 28 days post-challenge. IFNγ mRNA labelling appeared to be co-localised with Chlamydial lipopolysaccharide within trophoblast cells in discrete areas lining the primary villi in the limbus and hilar zone of the placentomes from challenged sheep on days 21 and 28 post-infection. The presence of IFNγ was also demonstrated by immunohistochemistry. No labelling was seen in tissues from the non-infected ewes. The presence of IFNγ in trophoblast cells from infected ewes may indicate an attempt to restrict the replication of the organism and be an important trigger for the inflammatory responses that develop on the fetal side of the placenta in enzootic abortion.

KW - Chlamydophila abortus

KW - In situ hybridization

KW - Interferon-gamma

KW - Ovine enzootic abortion

KW - Trophoblast cells

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U2 - 10.1016/j.jri.2011.03.011

DO - 10.1016/j.jri.2011.03.011

M3 - Article

C2 - 21722966

AN - SCOPUS:79960210324

SN - 0165-0378

VL - 90

SP - 214

EP - 219

JO - Journal of Reproductive Immunology

JF - Journal of Reproductive Immunology

IS - 2

ER -

Interferon-γ expression in trophoblast cells in pregnant ewes challenged with Chlamydophila abortus

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Keywords / Materials (for Non-textual outputs)

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