Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray

S A M Martin, J B Taggart, P Seear, J E Bron, R Talbot, A J Teale, G E Sweeney, B Høyheim, D F Houlihan, D R Tocher, J Zou, C J Secombes

Research output: Contribution to journalArticlepeer-review

Abstract

Interferons (IFNs) are cytokines that have proinflammatory, antiviral, and immunomodulatory effects and play a central role during a host response to pathogens. The IFN family contains both type I and type II molecules. While there are a number of type I IFNs, there is only one type II IFN. Recently both type I and type II IFN genes have been cloned in salmonid fish and recombinant proteins produced showing IFN activity. We have stimulated an Atlantic salmon cell line (SHK-1) with both type I and type II recombinant salmonid IFNs and analyzed the transcriptional response by microarray analysis. Cells were exposed to recombinant IFNs for 6 or 24 h or left unexposed as controls. RNA was hybridized to an Atlantic salmon cDNA microarray (salmon 17K feature TRAITS/SGP array) in order to assess differential gene expression in response to IFN exposure. For IFN I and II, 47 and 72 genes were stimulated, respectively; most genes were stimulated by a single IFN type, but some were affected by both IFNs, indicating coregulation of the IFN response in fish. Real-time PCR analysis was employed to confirm the microarray results for selected differentially expressed genes in both a cell line and primary leukocyte cultures.
Original languageEnglish
Pages (from-to)33-44
Number of pages12
JournalPhysiological Genomics
Volume32
Issue number1
DOIs
Publication statusPublished - 19 Dec 2007

Keywords

  • Animals
  • Cell Culture Techniques
  • Cell Line
  • Cloning, Molecular
  • Cytokines
  • DNA, Complementary
  • Interferon Type I
  • Interferon-gamma
  • Oligonucleotide Array Sequence Analysis
  • Recombinant Proteins
  • Salmo salar

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