Interneuron- and GABAA receptor-specific inhibitory synaptic plasticity in cerebellar purkinje cells

Qionger He, Ian Duguid, Beverley Clark, Patrizia Panzanelli, Bijal Patel, Philip Thomas, Jean-Marc Fritschy, Trevor G Smart

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Inhibitory synaptic plasticity is important for shaping both neuronal excitability and network activity. Here we investigate the input and GABAA receptor subunit specificity of inhibitory synaptic plasticity by studying cerebellar interneuron–Purkinje cell (PC) synapses. Depolarizing PCs initiated a long-lasting increase in GABA-mediated synaptic currents. By stimulating individual interneurons, this plasticity was observed at somatodendritic basket cell synapses, but not at distal dendritic stellate cell synapses. Basket cell synapses predominantly express β2-subunit-containing GABAA receptors; deletion of the β2-subunit ablates this plasticity, demonstrating its reliance on GABAA receptor subunit composition. The increase in synaptic currents is dependent upon an increase in newly synthesized cell surface synaptic GABAA receptors and is abolished by preventing CaMKII phosphorylation of GABAA receptors. Our results reveal a novel GABAA receptor subunit- and input-specific form of inhibitory synaptic plasticity that regulates the temporal firing pattern of the principal output cells of the cerebellum.
Original languageEnglish
Article number7364
JournalNature Communications
Volume6
DOIs
Publication statusPublished - 16 Jul 2015

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