Interphase cytogenetics using biotin and digoxigenin labelled probes: III. Increased sensitivity and flexibility for detecting HPV in cervical biopsy specimens and cell lines

C S Herrington, A K Graham, J O McGee

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

A monoclonal antibody to digoxin enabled sandwich techniques to be used for the detection of hybridised digoxigenin labelled probes in cultured cells and paraffin wax sections. This system has greater flexibility than alkaline phosphatase conjugated polyclonal antidigoxigenin antibody and permits the use of alternative detector enzymes, such as horseradish peroxidase and fluorescence labels. The APAAP detection system that does not require the use of biotin can also be used in situations where endogenous biotin is a problem. The low level of background staining combined with precise substrate deposition of the amplified peroxidase system gives higher sensitivity and resolution. This permits localisation of closely adjacent chromosomal loci in interphase nuclei. The most sensitive peroxidase based digoxigenin detection system visualises two and a half to 12 copies of human papillomavirus (HPV) per nucleus. This system is also suitable for the analysis of low copy number HPV infection of cervical tissues.

Original languageEnglish
Pages (from-to)33-8
Number of pages6
JournalJournal of Clinical Pathology
Volume44
Issue number1
Publication statusPublished - Jan 1991

Keywords / Materials (for Non-textual outputs)

  • Alkaline Phosphatase
  • Antibodies, Monoclonal
  • Biotin
  • Carcinoma in Situ
  • Cervix Uteri
  • Condylomata Acuminata
  • DNA Probes, HPV
  • Digoxigenin
  • Digoxin
  • Female
  • Fluorescent Antibody Technique
  • HeLa Cells
  • Humans
  • Interphase
  • Papillomaviridae
  • Uterine Cervical Neoplasms

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