Intracellular oxidation by human glioma cell populations: effect of arachidonic acid

H A Leaver; J R Williams; C Smith; I R Whittle

doi:10.1016/j.plefa.2003.09.005

Intracellular oxidation by human glioma cell populations: effect of arachidonic acid

H A Leaver, J R Williams, C Smith, I R Whittle

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Arachidonic acid (AA) and Gamma linolenic acid have been shown to limit glioma cell growth, stimulate apoptosis and lipid peroxidation. However, brain tumours are characterised by cellular heterogeneity and responding cell populations have not been identified. Brain tumour samples from patients were disaggregated. In cell preparations from 7 gliomas, reactive oxygen species (ROS), morphology and plasma membrane integrity were monitored +/-18-36 microM AA for 15-120 min using flow cytometry. Basal oxidative activity related to cell size/morphology, small granular cells showed lower activity. AA stimulation of ROS formation depended on cell size/morphology. Large, less granular cells showed greater AA stimulation. In 17 gliomas, GFAP immunofluorescence was demonstrated in larger cell populations. The large GFAP positive cell population with low side scatter was the highest responding cell population, suggesting selective tumour cell sensitivity to AA induced ROS formation. ROS may have a role in AA induced cell death and anti-tumour activity of AA in glioma.

Original language	English
Pages (from-to)	449-53
Number of pages	5
Journal	Prostaglandins, Leukotrienes and Essential Fatty Acids
Volume	70
Issue number	5
DOIs	https://doi.org/10.1016/j.plefa.2003.09.005
Publication status	Published - May 2004

Keywords / Materials (for Non-textual outputs)

Antineoplastic Agents
Arachidonic Acid
Brain Neoplasms
Cell Death
Cell Division
Glioma
Humans
Oxidation-Reduction
Reactive Oxygen Species
gamma-Linolenic Acid

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10.1016/j.plefa.2003.09.005

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@article{350b6756fbea4ff5b0c9a255ffa7f6da,

title = "Intracellular oxidation by human glioma cell populations: effect of arachidonic acid",

abstract = "Arachidonic acid (AA) and Gamma linolenic acid have been shown to limit glioma cell growth, stimulate apoptosis and lipid peroxidation. However, brain tumours are characterised by cellular heterogeneity and responding cell populations have not been identified. Brain tumour samples from patients were disaggregated. In cell preparations from 7 gliomas, reactive oxygen species (ROS), morphology and plasma membrane integrity were monitored +/-18-36 microM AA for 15-120 min using flow cytometry. Basal oxidative activity related to cell size/morphology, small granular cells showed lower activity. AA stimulation of ROS formation depended on cell size/morphology. Large, less granular cells showed greater AA stimulation. In 17 gliomas, GFAP immunofluorescence was demonstrated in larger cell populations. The large GFAP positive cell population with low side scatter was the highest responding cell population, suggesting selective tumour cell sensitivity to AA induced ROS formation. ROS may have a role in AA induced cell death and anti-tumour activity of AA in glioma.",

keywords = "Antineoplastic Agents, Arachidonic Acid, Brain Neoplasms, Cell Death, Cell Division, Glioma, Humans, Oxidation-Reduction, Reactive Oxygen Species, gamma-Linolenic Acid",

author = "Leaver, {H A} and Williams, {J R} and C Smith and Whittle, {I R}",

year = "2004",

month = may,

doi = "10.1016/j.plefa.2003.09.005",

language = "English",

volume = "70",

pages = "449--53",

journal = "Prostaglandins, Leukotrienes and Essential Fatty Acids",

issn = "0952-3278",

publisher = "Elsevier",

number = "5",

}

TY - JOUR

T1 - Intracellular oxidation by human glioma cell populations

T2 - effect of arachidonic acid

AU - Leaver, H A

AU - Williams, J R

AU - Smith, C

AU - Whittle, I R

PY - 2004/5

Y1 - 2004/5

N2 - Arachidonic acid (AA) and Gamma linolenic acid have been shown to limit glioma cell growth, stimulate apoptosis and lipid peroxidation. However, brain tumours are characterised by cellular heterogeneity and responding cell populations have not been identified. Brain tumour samples from patients were disaggregated. In cell preparations from 7 gliomas, reactive oxygen species (ROS), morphology and plasma membrane integrity were monitored +/-18-36 microM AA for 15-120 min using flow cytometry. Basal oxidative activity related to cell size/morphology, small granular cells showed lower activity. AA stimulation of ROS formation depended on cell size/morphology. Large, less granular cells showed greater AA stimulation. In 17 gliomas, GFAP immunofluorescence was demonstrated in larger cell populations. The large GFAP positive cell population with low side scatter was the highest responding cell population, suggesting selective tumour cell sensitivity to AA induced ROS formation. ROS may have a role in AA induced cell death and anti-tumour activity of AA in glioma.

AB - Arachidonic acid (AA) and Gamma linolenic acid have been shown to limit glioma cell growth, stimulate apoptosis and lipid peroxidation. However, brain tumours are characterised by cellular heterogeneity and responding cell populations have not been identified. Brain tumour samples from patients were disaggregated. In cell preparations from 7 gliomas, reactive oxygen species (ROS), morphology and plasma membrane integrity were monitored +/-18-36 microM AA for 15-120 min using flow cytometry. Basal oxidative activity related to cell size/morphology, small granular cells showed lower activity. AA stimulation of ROS formation depended on cell size/morphology. Large, less granular cells showed greater AA stimulation. In 17 gliomas, GFAP immunofluorescence was demonstrated in larger cell populations. The large GFAP positive cell population with low side scatter was the highest responding cell population, suggesting selective tumour cell sensitivity to AA induced ROS formation. ROS may have a role in AA induced cell death and anti-tumour activity of AA in glioma.

KW - Antineoplastic Agents

KW - Arachidonic Acid

KW - Brain Neoplasms

KW - Cell Death

KW - Cell Division

KW - Glioma

KW - Humans

KW - Oxidation-Reduction

KW - Reactive Oxygen Species

KW - gamma-Linolenic Acid

U2 - 10.1016/j.plefa.2003.09.005

DO - 10.1016/j.plefa.2003.09.005

M3 - Article

C2 - 15062847

SN - 0952-3278

VL - 70

SP - 449

EP - 453

JO - Prostaglandins, Leukotrienes and Essential Fatty Acids

JF - Prostaglandins, Leukotrienes and Essential Fatty Acids

IS - 5

ER -

Intracellular oxidation by human glioma cell populations: effect of arachidonic acid

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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