Abstract / Description of output
Background: Fine needle aspiration cytology (FNAC) of lymph nodes (LNs) is routinely used for staging canine malignant solid tumors, but studies evaluating its efficacy are limited.
Objectives: The primary objectives of this study were to evaluate the sensitivity/specificity of FNAC and the significance of non-diagnostic FNAC when staging canine malignant solid tumors. A secondary objective was to determine the frequency of multiple nodal metastases.
Methods: Histopathological and FNAC assessments of LNs (n = 259) draining malignant solid tumors were included. The sensitivity/specificity of FNAC was determined for 194 LNs with diagnostic FNAC, using histopathology as the gold standard. The proportion of non-diagnostic FNAC and associated histopathological prevalence of metastasis were determined. Among the tumors with multiple LNs assessed (88/189), the prevalence of multiple nodal metastases was determined.
Results: The sensitivity of FNAC was 67% for sarcomas, 100% for carcinomas, 63% for melanomas, 75% for mast cell tumors, and 100% for other round cell tumors. The specificity varied between 83% and 96%. Non-diagnostic FNAC was reported in 25% of LNs sampled, most of which were non-enlarged and/or difficult to access, and 20% of which were metastatic on histopathology. When several LNs were assessed, the prevalence of multiple nodal metastases was 24%.
Conclusions: Histopathologic LN evaluation cannot be robustly substituted with FNAC when staging selected canine solid tumors. When a diagnostic FNAC is elusive, as the prevalence of metastasis remains non-negligible in these cases, histopathological assessment is ideal. Finally, staging should not always be limited to the assessment of one single LN.
Objectives: The primary objectives of this study were to evaluate the sensitivity/specificity of FNAC and the significance of non-diagnostic FNAC when staging canine malignant solid tumors. A secondary objective was to determine the frequency of multiple nodal metastases.
Methods: Histopathological and FNAC assessments of LNs (n = 259) draining malignant solid tumors were included. The sensitivity/specificity of FNAC was determined for 194 LNs with diagnostic FNAC, using histopathology as the gold standard. The proportion of non-diagnostic FNAC and associated histopathological prevalence of metastasis were determined. Among the tumors with multiple LNs assessed (88/189), the prevalence of multiple nodal metastases was determined.
Results: The sensitivity of FNAC was 67% for sarcomas, 100% for carcinomas, 63% for melanomas, 75% for mast cell tumors, and 100% for other round cell tumors. The specificity varied between 83% and 96%. Non-diagnostic FNAC was reported in 25% of LNs sampled, most of which were non-enlarged and/or difficult to access, and 20% of which were metastatic on histopathology. When several LNs were assessed, the prevalence of multiple nodal metastases was 24%.
Conclusions: Histopathologic LN evaluation cannot be robustly substituted with FNAC when staging selected canine solid tumors. When a diagnostic FNAC is elusive, as the prevalence of metastasis remains non-negligible in these cases, histopathological assessment is ideal. Finally, staging should not always be limited to the assessment of one single LN.
Original language | English |
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Pages (from-to) | 489-500 |
Journal | Veterinary Clinical Pathology: An International Journal of Laboratory Medicine |
Volume | 47 |
Issue number | 3 |
Early online date | 16 Jul 2018 |
DOIs | |
Publication status | Published - Sept 2018 |
Keywords / Materials (for Non-textual outputs)
- Canine
- cytology
- lymphatic metastasis
- sensitivity
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Paola Cazzini
- Royal (Dick) School of Veterinary Studies - Senior Lecturer
Person: Academic: Research Active