Involvement of caveolae in the uptake of respiratory syncytial virus antigen by dendritic cells

Dirk Werling, Jayne Hope, Paul Chaplin, Robert A Collins, Geraldine Taylor, Chris J Howard

Research output: Contribution to journalArticlepeer-review


The uptake of respiratory syncytial virus (RSV) antigen by cattle dendritic cells was investigated. Pathways of antigen uptake were monitored by flow cytometry using specific tracers and by proliferation assays, which were used to measure the presentation of RSV antigen and ovalbumin. Inhibitors that differentially affected pathways were used to distinguish them. Presentation of RSV antigen, but not ovalbumin, was inhibited by phorbol myristate acetate and filipin, which have been reported to inhibit caveolae, but not by cytochalasin D, amiloride, or mannose. These inhibitors have been reported to block macropinocytosis and other actin-dependent uptake mechanisms, endocytic pathways involving clathrin-coated pits, and the mannose receptor. Furthermore, co-localization of RSV antigen and caveolae was observed by confocal microscopy. Thus, the major route for uptake of RSV antigen by cattle dendritic cells is one mediated by caveolae, adding a pathway of antigen uptake by dendritic cells to those established.
Original languageEnglish
Pages (from-to)50-8
Number of pages9
JournalJournal of Leukocyte Biology
Issue number1
Publication statusPublished - Jul 1999


  • Amiloride/pharmacology
  • Animals
  • Antigen Presentation
  • Antigens, Viral/metabolism
  • Brefeldin A/pharmacology
  • Cattle
  • Cell Membrane/metabolism
  • Clathrin/metabolism
  • Coated Pits, Cell-Membrane/metabolism
  • Cytochalasin D/pharmacology
  • Dendritic Cells/drug effects
  • Dendritic Cells/metabolism
  • Dendritic Cells/virology
  • Endocytosis
  • Filipin/pharmacology
  • Fluorescein-5-isothiocyanate/metabolism
  • Humans
  • Lectins, C-Type
  • Mannans/pharmacology
  • Mannose-Binding Lectins
  • Mice
  • Microscopy, Confocal
  • Ovalbumin/metabolism
  • Pinocytosis
  • Receptors, Cell Surface/metabolism
  • Respiratory Syncytial Viruses/metabolism
  • Tetradecanoylphorbol Acetate/pharmacology


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