Involvement of the lamin rod domain in heterotypic lamin interactions important for nuclear organization

Eric Schirmer, T Guan, L Gerace

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The nuclear lamina is a meshwork of intermediate-type filament proteins (lamins) that lines the inner nuclear membrane. The lamina is proposed to be an important determinant of nuclear structure, but there has been little direct testing of this idea. To investigate lamina functions, we have characterized a novel lamin B1 mutant lacking the middle approximately 4/5 of its alpha-helical rod domain. Though retaining only 10 heptads of the rod, this mutant assembles into intermediate filament-like structures in vitro. When expressed in cultured cells, it concentrates in patches at the nuclear envelope. Concurrently, endogenous lamins shift from a uniform to a patchy distribution and lose their complete colocalization, and nuclei become highly lobulated. In vitro binding studies suggest that the internal rod region is important for heterotypic associations of lamin B1, which in turn are required for proper organization of the lamina. Accompanying the changes in lamina structure induced by expression of the mutant, nuclear pore complexes and integral membrane proteins of the inner membrane cluster, principally at the patches of endogenous lamins. Considered together, these data indicate that lamins play a major role in organizing other proteins in the nuclear envelope and in determining nuclear shape.
Original languageEnglish
Pages (from-to)479-89
Number of pages11
JournalJournal of Cell Biology
Issue number3
Publication statusPublished - 2001

Keywords / Materials (for Non-textual outputs)

  • Animals
  • COS Cells
  • Cell Nucleus
  • HeLa Cells
  • Humans
  • Intermediate Filament Proteins
  • Intermediate Filaments
  • Lamin Type B
  • Lamins
  • Mutation
  • Nuclear Envelope
  • Nuclear Pore
  • Nuclear Proteins
  • Protein Binding
  • Protein Engineering
  • Protein Structure, Tertiary
  • Recombinant Proteins
  • Sequence Deletion


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