Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores

Emma M J King, Najma Rachidi, Nick Morrice, Kevin G Hardwick, Michael J R Stark

Research output: Contribution to journalArticlepeer-review

Abstract

The spindle checkpoint delays anaphase onset until all chromosomes are correctly attached to microtubules. Ipl1 protein kinase (Aurora B) is required to correct inappropriate kinetochore-microtubule attachments and for the response to lack of tension between sister kinetochores. Here we identify residues in the checkpoint protein Mad3p that are phosphorylated by Ipl1p. When phosphorylation of Mad3p at two sites is prevented, the cell's response to reduced kinetochore tension is dramatically curtailed. Our data provide strong evidence for a distinct checkpoint pathway responding to lack of sister kinetochore tension, in which Ipl1p-dependent phosphorylation of Mad3p is a key step.
Original languageEnglish
Pages (from-to)1163-8
Number of pages6
JournalGenes & Development
Volume21
Issue number10
DOIs
Publication statusPublished - 2007

Keywords

  • Mad3
  • Aurora kinase
  • tension
  • spindle checkpoint

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