Isolating single primary rat hippocampal neurons & astrocytes on ultra-thin patterned parylene-C/silicon dioxide substrates

Charles R. Unsworth, Evangelos Delivopoulos, Trudi Gillespie, Alan F. Murray

Research output: Contribution to journalArticlepeer-review

Abstract

We report here the patterning of primary rat neurons and astrocytes from the postnatal hippocampus on ultra-thin parylene-C deposited on a silicon dioxide substrate, following observations of neuronal, astrocytic and nuclear coverage on strips of different lengths, widths and thicknesses. Neuronal and glial growth was characterized 'on', 'adjacent to' and 'away from' the parylene strips. In addition, the article reports how the same material combination can be used to isolate single cells along thin tracks of parylene-C. This is demonstrated with a series of high magnification images of the experimental observations for varying parylene strip widths and thicknesses. Thus, the findings demonstrate the possibility to culture cells on ultra-thin layers of parylene-C and localize single cells on thin strips. Such work is of interest and significance to the Neuroengineering and Multi-Electrode Array (MEA) communities, as it provides an alternative insulating material in the fabrication of embedded micro-electrodes, which can be used to facilitate single cell stimulation and recording in capacitive coupling mode. (C) 2010 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)2566-2574
Number of pages9
JournalBiomaterials
Volume32
Issue number10
DOIs
Publication statusPublished - Apr 2011

Keywords

  • Cell patterning
  • Primary rat cell
  • Neuron
  • Astrocyte
  • Parylene-C
  • Brain cell
  • EXTRACELLULAR STIMULATION
  • SILICON CHIP
  • NERVE-CELLS
  • CULTURE
  • GROWTH
  • GLIA

Fingerprint

Dive into the research topics of 'Isolating single primary rat hippocampal neurons & astrocytes on ultra-thin patterned parylene-C/silicon dioxide substrates'. Together they form a unique fingerprint.

Cite this