Isolation and characterization of 102 new microsatellite loci in Murray cod, Maccullochella peelii peelii (Percichthyidae), and assessment of cross-amplification in 13 Australian native and six introduced freshwater species

Meaghan Rourke; Jenny Nheu; Hayley Mountford; Jennifer Lade; Brett Ingram; Helen McPartlan

doi:10.1111/j.1471-8286.2007.01849.x

Isolation and characterization of 102 new microsatellite loci in Murray cod, Maccullochella peelii peelii (Percichthyidae), and assessment of cross-amplification in 13 Australian native and six introduced freshwater species

Meaghan Rourke^*, Jenny Nheu, Hayley Mountford, Jennifer Lade, Brett Ingram, Helen McPartlan

^*Corresponding author for this work

School of Philosophy, Psychology and Language Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

We have isolated 102 polymorphic microsatellite loci from an enriched Murray cod DNA library and also assessed their amplification success in 13 native and six introduced freshwater fish species. The loci will serve the dual purpose of assessing wild population genetic structure for future conservation efforts, and for identifying markers for key quantitative trait loci important for aquaculture.

Original language	English
Pages (from-to)	1258-1264
Number of pages	7
Journal	Molecular Ecology Notes
Volume	7
Issue number	6
Early online date	2 Jun 2007
DOIs	https://doi.org/10.1111/j.1471-8286.2007.01849.x
Publication status	Published - Nov 2007

Keywords / Materials (for Non-textual outputs)

aquaculture
microsatellites
Murray cod
Percicthyidae
population structure
Terapontidae

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10.1111/j.1471-8286.2007.01849.x

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Rourke, M., Nheu, J., Mountford, H., Lade, J., Ingram, B., & McPartlan, H. (2007). Isolation and characterization of 102 new microsatellite loci in Murray cod, Maccullochella peelii peelii (Percichthyidae), and assessment of cross-amplification in 13 Australian native and six introduced freshwater species. Molecular Ecology Notes, 7(6), 1258-1264. https://doi.org/10.1111/j.1471-8286.2007.01849.x

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title = "Isolation and characterization of 102 new microsatellite loci in Murray cod, Maccullochella peelii peelii (Percichthyidae), and assessment of cross-amplification in 13 Australian native and six introduced freshwater species",

abstract = "We have isolated 102 polymorphic microsatellite loci from an enriched Murray cod DNA library and also assessed their amplification success in 13 native and six introduced freshwater fish species. The loci will serve the dual purpose of assessing wild population genetic structure for future conservation efforts, and for identifying markers for key quantitative trait loci important for aquaculture.",

keywords = "aquaculture, microsatellites, Murray cod, Percicthyidae, population structure, Terapontidae",

author = "Meaghan Rourke and Jenny Nheu and Hayley Mountford and Jennifer Lade and Brett Ingram and Helen McPartlan",

note = "Acknowledgements: We acknowledge the Victorian Government's Our Rural Landscape Initiative, Fisheries Victoria and the Holsthworth Wildlife Research Fund for project funding, and the Commonwealth Government for providing an Australian Postgraduate Award to Meaghan Rourke. We thank Andrea Taylor for cosupervision, Keith Savin and Jovana Maksimovic for bioinformatic analysis and Nick Robinson for advice with statistical analyses. Samples of other species were donated by Stuart Rowland, Catherine Nock, Bob Simpson and Janet Pritchard.",

year = "2007",

month = nov,

doi = "10.1111/j.1471-8286.2007.01849.x",

language = "English",

volume = "7",

pages = "1258--1264",

journal = "Molecular Ecology Notes",

issn = "1471-8278",

publisher = "Wiley-Blackwell",

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Rourke, M, Nheu, J, Mountford, H, Lade, J, Ingram, B & McPartlan, H 2007, 'Isolation and characterization of 102 new microsatellite loci in Murray cod, Maccullochella peelii peelii (Percichthyidae), and assessment of cross-amplification in 13 Australian native and six introduced freshwater species', Molecular Ecology Notes, vol. 7, no. 6, pp. 1258-1264. https://doi.org/10.1111/j.1471-8286.2007.01849.x

Isolation and characterization of 102 new microsatellite loci in Murray cod, Maccullochella peelii peelii (Percichthyidae), and assessment of cross-amplification in 13 Australian native and six introduced freshwater species. / Rourke, Meaghan; Nheu, Jenny; Mountford, Hayley et al.
In: Molecular Ecology Notes, Vol. 7, No. 6, 11.2007, p. 1258-1264.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Isolation and characterization of 102 new microsatellite loci in Murray cod, Maccullochella peelii peelii (Percichthyidae), and assessment of cross-amplification in 13 Australian native and six introduced freshwater species

AU - Rourke, Meaghan

AU - Nheu, Jenny

AU - Mountford, Hayley

AU - Lade, Jennifer

AU - Ingram, Brett

AU - McPartlan, Helen

N1 - Acknowledgements: We acknowledge the Victorian Government's Our Rural Landscape Initiative, Fisheries Victoria and the Holsthworth Wildlife Research Fund for project funding, and the Commonwealth Government for providing an Australian Postgraduate Award to Meaghan Rourke. We thank Andrea Taylor for cosupervision, Keith Savin and Jovana Maksimovic for bioinformatic analysis and Nick Robinson for advice with statistical analyses. Samples of other species were donated by Stuart Rowland, Catherine Nock, Bob Simpson and Janet Pritchard.

PY - 2007/11

Y1 - 2007/11

N2 - We have isolated 102 polymorphic microsatellite loci from an enriched Murray cod DNA library and also assessed their amplification success in 13 native and six introduced freshwater fish species. The loci will serve the dual purpose of assessing wild population genetic structure for future conservation efforts, and for identifying markers for key quantitative trait loci important for aquaculture.

AB - We have isolated 102 polymorphic microsatellite loci from an enriched Murray cod DNA library and also assessed their amplification success in 13 native and six introduced freshwater fish species. The loci will serve the dual purpose of assessing wild population genetic structure for future conservation efforts, and for identifying markers for key quantitative trait loci important for aquaculture.

KW - aquaculture

KW - microsatellites

KW - Murray cod

KW - Percicthyidae

KW - population structure

KW - Terapontidae

U2 - 10.1111/j.1471-8286.2007.01849.x

DO - 10.1111/j.1471-8286.2007.01849.x

M3 - Article

AN - SCOPUS:35848956826

SN - 1471-8278

VL - 7

SP - 1258

EP - 1264

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 6

ER -

Rourke M, Nheu J, Mountford H, Lade J, Ingram B, McPartlan H. Isolation and characterization of 102 new microsatellite loci in Murray cod, Maccullochella peelii peelii (Percichthyidae), and assessment of cross-amplification in 13 Australian native and six introduced freshwater species. Molecular Ecology Notes. 2007 Nov;7(6):1258-1264. Epub 2007 Jun 2. doi: 10.1111/j.1471-8286.2007.01849.x