Isolation and characterization of canine perivascular stem/stromal cells for bone tissue engineering

Aaron W James, Xinli Zhang, Mihaela Crisan, Winters R Hardy, Pei Liang, Carolyn A Meyers, Sonja Lobo, Venu Lagishetty, Martin K Childers, Greg Asatrian, Catherine Ding, Yu-Hsin Yen, Erin Zou, Kang Ting, Bruno Peault, Chia Soo

Research output: Contribution to journalArticlepeer-review

Abstract

For over 15 years, human subcutaneous adipose tissue has been recognized as a rich source of tissue resident mesenchymal stem/stromal cells (MSC). The isolation of perivascular progenitor cells from human adipose tissue by a cell sorting strategy was first published in 2008. Since this time, the interest in using pericytes and related perivascular stem/stromal cell (PSC) populations for tissue engineering has significantly increased. Here, we describe a set of experiments identifying, isolating and characterizing PSC from canine tissue (N = 12 canine adipose tissue samples). Results showed that the same antibodies used for human PSC identification and isolation are cross-reactive with canine tissue (CD45, CD146, CD34). Like their human correlate, canine PSC demonstrate characteristics of MSC including cell surface marker expression, colony forming unit-fibroblast (CFU-F) inclusion, and osteogenic differentiation potential. As well, canine PSC respond to osteoinductive signals in a similar fashion as do human PSC, such as the secreted differentiation factor NEL-Like Molecule-1 (NELL-1). Nevertheless, important differences exist between human and canine PSC, including differences in baseline osteogenic potential. In summary, canine PSC represent a multipotent mesenchymogenic cell source for future translational efforts in tissue engineering.

Original languageEnglish
Pages (from-to)e0177308
JournalPLoS ONE
Volume12
Issue number5
DOIs
Publication statusPublished - 10 May 2017

Keywords

  • Journal Article

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