Isolation and characterization of highly polymorphic microsatellite loci in the 2-spot ladybird, Adalia bipunctata

P R  Haddrill; M E N  Majerus; S  Mayes

doi:10.1046/j.1471-8278.2002.00227.x

Isolation and characterization of highly polymorphic microsatellite loci in the 2-spot ladybird, Adalia bipunctata

P R Haddrill, M E N Majerus, S Mayes

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Contrary to theoretical predictions, female 2-spot ladybirds (Adalia bipunctata) mate many more times than necessary to maintain high fertilisation success and may gain through the acquisition of material or genetic benefits. In order to investigate this mating system in detail, microsatellite markers have been isolated using a modified enrichment technique. Thirty-nine loci were successfully amplified by polymerase chain reaction (PCR), of which only two were monomorphic. Detailed characterization of ten loci revealed very high levels of polymorphism. These markers are likely to be invaluable tools with which to study population genetics and patterns of paternity in this species.

Original language	English
Pages (from-to)	316-319
Number of pages	4
Journal	Molecular Ecology Notes
Volume	2
Issue number	3
DOIs	https://doi.org/10.1046/j.1471-8278.2002.00227.x
Publication status	Published - Sept 2002

Access to Document

10.1046/j.1471-8278.2002.00227.x

Cite this

@article{b9b73d3816eb4085bca0d66628bcadb1,

title = "Isolation and characterization of highly polymorphic microsatellite loci in the 2-spot ladybird, Adalia bipunctata",

abstract = "Contrary to theoretical predictions, female 2-spot ladybirds (Adalia bipunctata) mate many more times than necessary to maintain high fertilisation success and may gain through the acquisition of material or genetic benefits. In order to investigate this mating system in detail, microsatellite markers have been isolated using a modified enrichment technique. Thirty-nine loci were successfully amplified by polymerase chain reaction (PCR), of which only two were monomorphic. Detailed characterization of ten loci revealed very high levels of polymorphism. These markers are likely to be invaluable tools with which to study population genetics and patterns of paternity in this species.",

author = "Haddrill, {P R} and Majerus, {M E N} and S Mayes",

year = "2002",

month = sep,

doi = "10.1046/j.1471-8278.2002.00227.x",

language = "English",

volume = "2",

pages = "316--319",

journal = "Molecular Ecology Notes",

issn = "1471-8278",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Isolation and characterization of highly polymorphic microsatellite loci in the 2-spot ladybird, Adalia bipunctata

AU - Haddrill, P R

AU - Majerus, M E N

AU - Mayes, S

PY - 2002/9

Y1 - 2002/9

N2 - Contrary to theoretical predictions, female 2-spot ladybirds (Adalia bipunctata) mate many more times than necessary to maintain high fertilisation success and may gain through the acquisition of material or genetic benefits. In order to investigate this mating system in detail, microsatellite markers have been isolated using a modified enrichment technique. Thirty-nine loci were successfully amplified by polymerase chain reaction (PCR), of which only two were monomorphic. Detailed characterization of ten loci revealed very high levels of polymorphism. These markers are likely to be invaluable tools with which to study population genetics and patterns of paternity in this species.

AB - Contrary to theoretical predictions, female 2-spot ladybirds (Adalia bipunctata) mate many more times than necessary to maintain high fertilisation success and may gain through the acquisition of material or genetic benefits. In order to investigate this mating system in detail, microsatellite markers have been isolated using a modified enrichment technique. Thirty-nine loci were successfully amplified by polymerase chain reaction (PCR), of which only two were monomorphic. Detailed characterization of ten loci revealed very high levels of polymorphism. These markers are likely to be invaluable tools with which to study population genetics and patterns of paternity in this species.

U2 - 10.1046/j.1471-8278.2002.00227.x

DO - 10.1046/j.1471-8278.2002.00227.x

M3 - Article

SN - 1471-8278

VL - 2

SP - 316

EP - 319

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 3

ER -

Isolation and characterization of highly polymorphic microsatellite loci in the 2-spot ladybird, Adalia bipunctata

Abstract / Description of output

Access to Document

Fingerprint

Cite this